CPT1A Antibody - middle region (ARP44796_P050)

Catalog No: ARP44796_P050

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 5 ug/mL of the antibody was used in this experiment. Recommended dilution for this antibody is 1-3 ug/mL.

Sample Type:
Human Capan1 cells (Pancreatic cancer cell line)
Primary Antibody Dilution:
1:300
Secondary Antibody:
Anti-rabbit-AlexaFluor-488
Secondary Antibody Dilution:
1:200
Color/Signal Descriptions:
CPT1A: Green DAPI: Blue
Gene Name:
CPT1A
Submitted by:
Dr. Pankaj Singh, UNMC, Omaha, NE

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CPT1A
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: LSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGYGVSYILVGENLIN
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CPT1A (ARP44796_P050) antibody is Catalog # AAP44796 (Previous Catalog # AAPP12272)
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Reference: Roomets,E., (2008) Invest. Ophthalmol. Vis. Sci. 49 (4), 1660-1664
• Publications:

  Laghezza, A. et al. New 2-(aryloxy)-3-phenylpropanoic acids as peroxisome proliferator-activated receptor a/γ dual agonists able to upregulate mitochondrial carnitine shuttle system gene expression. J. Med. Chem. 56, 60-72 (2013). 23171045

  New diphenylmethane derivatives as peroxisome proliferator-activated receptor alpha/gamma dual agonists endowed with anti-proliferative effects and mitochondrial activity. Eur J Med Chem. 127, 379-397 (2017). 28076827

  Silibinin improves nonalcoholic fatty liver by regulating the expression of miR‑122: An in vitro and in vivo study. Mol Med Rep. 23 (2021) 33760189

  The contribution of the citrate pathway to oxidative stress in Down syndrome. Immunology. 149, 423-431 (2016). 27502741

  The role of CD36-Fabp4-PPARγ in skeletal muscle involves insulin resistance in intrauterine growth retardation mice with catch-up growth. BMC Endocr Disord. 22, 10 (2022). 34983495

Target Info

• Gene Symbol: CPT1A
• Gene Full Name: Carnitine palmitoyltransferase 1A (liver)
• Alias Symbols: CPT1, CPT1-L, L-CPT1
• NCBI Gene Id: 1374
• Protein Name: Carnitine O-palmitoyltransferase 1, liver isoform
• Description of Target: The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P50416
• Protein Accession #: NP_001027017
• Nucleotide Accession #: NM_001031847
• Protein Size (# AA): 756
• Molecular Weight: 88 kDa
• Protein Interactions: UBC; SUMO2; LGR4; RNF2; FBXO6; PARK2; LYN; CLN3; HDAC11; KBTBD7; NPDC1; HSPA5; NR4A1; CYBA; CLIC1; SNRNP200; ERLIN1; TOMM40; EIF3C; PCBP1; NDUFV1; NDUFS1; NDUFA2; LPP; CPS1; ATP6V1C1; RHOA; PCK1; BCL2;