Product Number |
ARP44796_P050 |
Product Page |
www.avivasysbio.com/cpt1a-antibody-middle-region-arp44796-p050.html |
Name |
CPT1A Antibody - middle region (ARP44796_P050) |
Protein Size (# AA) |
756 amino acids |
Molecular Weight |
88 kDa |
NCBI Gene Id |
1374 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Carnitine palmitoyltransferase 1A (liver) |
Description |
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Alias Symbols |
CPT1, CPT1-L, L-CPT1 |
Peptide Sequence |
Synthetic peptide located within the following region: LSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGYGVSYILVGENLIN |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Roomets,E., (2008) Invest. Ophthalmol. Vis. Sci. 49 (4), 1660-1664 |
Description of Target |
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Protein Interactions |
UBC; SUMO2; LGR4; RNF2; FBXO6; PARK2; LYN; CLN3; HDAC11; KBTBD7; NPDC1; HSPA5; NR4A1; CYBA; CLIC1; SNRNP200; ERLIN1; TOMM40; EIF3C; PCBP1; NDUFV1; NDUFS1; NDUFA2; LPP; CPS1; ATP6V1C1; RHOA; PCK1; BCL2; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Enhanced Validation |
Relative Expression (Western Blot) |
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Datasheets/Manuals |
Printable datasheet for anti-CPT1A (ARP44796_P050) antibody |
Blocking Peptide |
For anti-CPT1A (ARP44796_P050) antibody is Catalog # AAP44796 (Previous Catalog # AAPP12272) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human CPT1A |
Uniprot ID |
P50416 |
Protein Name |
Carnitine O-palmitoyltransferase 1, liver isoform |
Publications |
Laghezza, A. et al. New 2-(aryloxy)-3-phenylpropanoic acids as peroxisome proliferator-activated receptor a/γ dual agonists able to upregulate mitochondrial carnitine shuttle system gene expression. J. Med. Chem. 56, 60-72 (2013). 23171045
New diphenylmethane derivatives as peroxisome proliferator-activated receptor alpha/gamma dual agonists endowed with anti-proliferative effects and mitochondrial activity. Eur J Med Chem. 127, 379-397 (2017). 28076827
Silibinin improves nonalcoholic fatty liver by regulating the expression of miRâ122: An in vitro and in vivo study. Mol Med Rep. 23 (2021) 33760189
The contribution of the citrate pathway to oxidative stress in Down syndrome. Immunology. 149, 423-431 (2016). 27502741
The role of CD36-Fabp4-PPARγ in skeletal muscle involves insulin resistance in intrauterine growth retardation mice with catch-up growth. BMC Endocr Disord. 22, 10 (2022). 34983495 |
Protein Accession # |
NP_001027017 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_001031847 |
Tested Species Reactivity |
Human |
Gene Symbol |
CPT1A |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100% |
Image 1 | Human Capan1
| Sample Type: Human Capan1 cells (Pancreatic cancer cell line)Primary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-AlexaFluor-488Secondary Antibody Dilution: 1:200Color/Signal Descriptions: CPT1A: Green DAPI: BlueGene Name: CPT1ASubmitted by: Dr. Pankaj Singh, UNMC, Omaha, NE |
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Image 2 | Western Blot
| 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 5 ug/mL of the antibody was used in this experiment. Recommended dilution for this antibody is 1-3 ug/mL. |
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