CPT1A Antibody - middle region (ARP44796_P050)

Data Sheet
 
Product Number ARP44796_P050
Product Page www.avivasysbio.com/cpt1a-antibody-middle-region-arp44796-p050.html
Name CPT1A Antibody - middle region (ARP44796_P050)
Protein Size (# AA) 756 amino acids
Molecular Weight 88 kDa
NCBI Gene Id 1374
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Carnitine palmitoyltransferase 1A (liver)
Description
Alias Symbols CPT1, CPT1-L, L-CPT1
Peptide Sequence Synthetic peptide located within the following region: LSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGYGVSYILVGENLIN
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Roomets,E., (2008) Invest. Ophthalmol. Vis. Sci. 49 (4), 1660-1664
Description of Target The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Protein Interactions UBC; SUMO2; LGR4; RNF2; FBXO6; PARK2; LYN; CLN3; HDAC11; KBTBD7; NPDC1; HSPA5; NR4A1; CYBA; CLIC1; SNRNP200; ERLIN1; TOMM40; EIF3C; PCBP1; NDUFV1; NDUFS1; NDUFA2; LPP; CPS1; ATP6V1C1; RHOA; PCK1; BCL2;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
Datasheets/Manuals Printable datasheet for anti-CPT1A (ARP44796_P050) antibody
Blocking Peptide For anti-CPT1A (ARP44796_P050) antibody is Catalog # AAP44796 (Previous Catalog # AAPP12272)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CPT1A
Uniprot ID P50416
Protein Name Carnitine O-palmitoyltransferase 1, liver isoform
Publications

Laghezza, A. et al. New 2-(aryloxy)-3-phenylpropanoic acids as peroxisome proliferator-activated receptor a/γ dual agonists able to upregulate mitochondrial carnitine shuttle system gene expression. J. Med. Chem. 56, 60-72 (2013). 23171045

New diphenylmethane derivatives as peroxisome proliferator-activated receptor alpha/gamma dual agonists endowed with anti-proliferative effects and mitochondrial activity. Eur J Med Chem. 127, 379-397 (2017). 28076827

Silibinin improves nonalcoholic fatty liver by regulating the expression of miR‑122: An in vitro and in vivo study. Mol Med Rep. 23 (2021) 33760189

The contribution of the citrate pathway to oxidative stress in Down syndrome. Immunology. 149, 423-431 (2016). 27502741

The role of CD36-Fabp4-PPARγ in skeletal muscle involves insulin resistance in intrauterine growth retardation mice with catch-up growth. BMC Endocr Disord. 22, 10 (2022). 34983495

Protein Accession # NP_001027017
Purification Affinity Purified
Nucleotide Accession # NM_001031847
Tested Species Reactivity Human
Gene Symbol CPT1A
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
Image 1
Human Capan1
Sample Type:
Human Capan1 cells (Pancreatic cancer cell line)
Primary Antibody Dilution:
1:300
Secondary Antibody:
Anti-rabbit-AlexaFluor-488
Secondary Antibody Dilution:
1:200
Color/Signal Descriptions:
CPT1A: Green DAPI: Blue
Gene Name:
CPT1A
Submitted by:
Dr. Pankaj Singh, UNMC, Omaha, NE
Image 2
Western Blot
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 5 ug/mL of the antibody was used in this experiment. Recommended dilution for this antibody is 1-3 ug/mL.
 

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