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Catalog No: OKEH00404
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CPT1A ELISA Kit (Human) (OKEH00404) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CPT1A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human CPT1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CPT1A captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.7% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
Anti-GSN Microplate96 Wells (12 x 8 Well strips)
GSN Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated GSN1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Carnitine O-palmitoyltransferase 1, liver isoform

Snail augments fatty acid oxidation by suppression of mitochondrial ACC2 during cancer progression. Life Sci Alliance. 3, NULL (2020). 32487689

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCPT1A
Gene Full Namecarnitine palmitoyltransferase 1A
Alias Symbolscarnitine O-palmitoyltransferase 1, liver isoform;Carnitine O-palmitoyltransferase I, liver isoform;Carnitine palmitoyltransferase 1A;carnitine palmitoyltransferase 1A (liver);carnitine palmitoyltransferase I, liver;CPT I;CPT1;CPT1-L;CPTI-L;L-CPT1.
NCBI Gene Id1374
Protein NameP50416
Description of TargetCarnitine O-palmitoyltransferase 1, liver isoform
Uniprot ID
Protein Accession #NP_001027017.1
Nucleotide Accession #NM_001031847.2
Protein Size (# AA)773
Molecular Weight88 kDa
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