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- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for anti-CHRNA4 antibody - OAEB02107 |
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| Predicted Species Reactivity | Human, Mouse, Rat, Cow, Guinea Pig, Rabbit |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | WB |
| :: | Description: This antibody is expected NOT to cross-react with the similar alpha 2 subunit. |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | Peptide with sequence C-HVETRAHAEERLLKK, from the internal region of the protein sequence according to NP_000735.1. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Predicted Homology Based on Immunogen Sequence | Cow: 93%; Guinea Pig: 92%; Human: 100%; Mouse: 92%; Rabbit: 91%; Rat: 92% |
| Peptide Sequence | C-HVETRAHAEERLLKK |
| Application Info | Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. |
| Reference | * Schwarz J, Schwarz SC, Dorigo O, Stutzer A, Wegner F, Labarca C, Deshpande P, Gil JS, Berk AJ, Lester HA.; Enhanced expression of hypersensitive alpha4* nAChR in adult mice increases the loss of midbrain dopaminergic neurons.; FASEB J. 2006 May;20(7):935-46.; Pubmed 16675851 |
| Gene Symbol | CHRNA4 |
|---|---|
| Alias Symbols | EBN, BFNC, EBN1, NACHR, NACRA4, NACHRA4 |
| NCBI Gene Id | 1137 |
| Protein Name | Neuronal acetylcholine receptor subunit alpha-4 |
| Uniprot ID | P43681 |
| Protein Accession # | NP_000735.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CHRNA4 Antibody (OAEB02107)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Guinea Pig, Rabbit".
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How long will it take to receive "CHRNA4 Antibody (OAEB02107)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
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What buffer format is "CHRNA4 Antibody (OAEB02107)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CHRNA4 Antibody (OAEB02107)"?
This target may also be called "EBN, BFNC, EBN1, NACHR, NACRA4, NACHRA4" in publications.
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What is the shipping cost for "CHRNA4 Antibody (OAEB02107)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CHRNA4 Antibody (OAEB02107)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CHRNA4 Antibody (OAEB02107)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CHRNA4 Antibody (OAEB02107)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CHRNA4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CHRNA4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CHRNA4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CHRNA4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CHRNA4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CHRNA4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
