| Product Number |
OAEB02107 |
| Product Page |
www.avivasysbio.com/chrna4-antibody-oaeb02107.html |
| Name |
CHRNA4 Antibody (OAEB02107) |
| Molecular Weight |
70 |
| NCBI Gene Id |
1137|11438|25590 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
cholinergic receptor nicotinic alpha 4 subunit|cholinergic receptor, nicotinic, alpha polypeptide 4 |
| Alias Symbols |
a4 nicotinic receptor;Ac;acetylcholine receptor alpha 4 neural;acetylcholine receptor, neuronal, nicotinic, alpha-4 subunit;Acr;Acra4;Acra-4;alpha4 nAChR;BFNC;cholinergic receptor, nicotinic alpha 4;cholinergic receptor, nicotinic, alpha 4 (neuronal);cholinergic receptor, nicotinic, alpha polypeptide 4;EBN;EBN1;ENFL1;NACHR;NACHRA4;NACRA4;NARAC;neuronal acetylcholine receptor subunit alpha-4;Neuronal nicotinic acetylcholine receptor alpha 4 subunit;neuronal nicotinic acetylcholine receptor alpha-4 subunit. |
| Peptide Sequence |
C-HVETRAHAEERLLKK |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Schwarz J, Schwarz SC, Dorigo O, Stutzer A, Wegner F, Labarca C, Deshpande P, Gil JS, Berk AJ, Lester HA.; Enhanced expression of hypersensitive alpha4* nAChR in adult mice increases the loss of midbrain dopaminergic neurons.; FASEB J. 2006 May;20(7):935-46.; Pubmed 16675851 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for CHRNA4 Antibody (OAEB02107) |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:64000.
Western Blot: Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. |
| Immunogen |
CHRNA4 |
| Additional Information |
Description: This antibody is expected NOT to cross-react with the similar alpha 2 subunit. |
| Uniprot ID |
P43681 |
| Protein Name |
Neuronal acetylcholine receptor subunit alpha-4 |
| Protein Accession # |
NP_000735.1 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
Chrna4|CHRNA4 |
| Predicted Species Reactivity |
Bovine|Dog|Human|Mouse|Rat |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Guinea Pig: 92%; Human: 100%; Mouse: 92%; Rabbit: 91%; Rat: 92% |
| Image 1 | Rat Brain
 | OAEB02107 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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