Catalog No: OKEH05302
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology CHRNA4 ELISA Kit (Human) (OKEH05302) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHRNA4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHRNA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHRNA4 captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 101%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <5.5%, n = 20
Mean Inter-Assay Precision - <6.8%, n = 20
ELISA Kit Component
CHRNA4 Microplate96 Wells (12 x 8 Well strips)
CHRNA4 Lyophilized Standard2
100X Biotinylated CHRNA4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity39.7 pg/mL
SpecificityNatural and recombinant Human Neuronal acetylcholine receptor subunit alpha-4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCHRNA4
Gene Full Namecholinergic receptor nicotinic alpha 4 subunit
Alias SymbolsBFNC;cholinergic receptor, nicotinic alpha 4;cholinergic receptor, nicotinic, alpha 4 (neuronal);cholinergic receptor, nicotinic, alpha polypeptide 4;EBN;EBN1;NACHR;NACHRA4;NACRA4;neuronal acetylcholine receptor subunit alpha-4;neuronal nicotinic acetylcholine receptor alpha-4 subunit.
NCBI Gene Id1137
Protein NameP43681
Description of TargetNeuronal acetylcholine receptor subunit alpha-4
Uniprot ID
Protein Accession #NP_000735.1
Nucleotide Accession #NM_000744.6
Protein Size (# AA)627
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