CD79A Antibody (OAEE00314)

Catalog No: OAEE00314

Size:100UG

Intracellular staining of CD79a with anti-CD79a (HM57) PE (gated on leukemic blast cells) in a patient with childhood B-precursor ALL.

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Guinea Pig|Horse|Human|Mouse|Opossum|Porcine|Rabbit|Rat
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: HM57
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Synthetic peptide corresponding to amino acids 202-216 of human CD79a
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody HM57 interacts with CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
  HLDA V; WS Code BC cB018
  HLDA VI; WS Code BP 193
  HLDA VI; WS Code BP 89
  HLDA VI; WS Code B B103
  HLDA VI; WS Code B CD79.4
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):56-62.
  Mason DY, van Noesel CJ, Cordell JL, Comans-Bitter WM, Micklem K, Tse AG, van Lier RA, van Dongen JJ: The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation. Eur J Immunol. 1992 Oct;22(10):2753-6.
  van Noesel CJ, van Lier RA, Cordell JL, Tse AG, van Schijndel GM, de Vries EF, Mason DY, Borst J: The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene. J Immunol. 1991 Jun 1;146(11):3881-8.
  Jones M, Cordell JL, Beyers AD, Tse AG, Mason DY: Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J Immunol. 1993 Jun 15;150(12):5429-35.
  Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, Jaffe E, Ralfkiaer E, Dallenbach F, Stein H, et al: CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood. 1995 Aug 15;86(4):1453-9.
  Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147(11):2474-82.
  Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe JG: Analysis of the individual contributions of Igalpha (CD79a)- and Igbeta (CD79b)-mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J Immunol. 2006 Dec 1;177(11):7913-22.
  Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174(3):1245-52.
  Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173(2):1000-11.
  Pike KA, Iacampo S, Friedmann JE, Ratcliffe MJ: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15;172(4):2210-8.
  Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe JG: Ligand-independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J Exp Med. 2001 Dec 3;194(11):1583-96.

Target Info

• Gene Symbol: CD79A
• Gene Full Name: CD79a molecule
• Alias Symbols: B-cell antigen receptor complex-associated protein alpha chain;CD79a antigen (immunoglobulin-associated alpha);CD79a molecule, immunoglobulin-associated alpha;IGA;ig-alpha;MB-1;MB-1 membrane glycoprotein;membrane-bound immunoglobulin-associated protein;surface IgM-associated protein.
• NCBI Gene Id: 973
• Protein Name: B-cell antigen receptor complex-associated protein alpha chain
• Description of Target: Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.
• Uniprot ID: P11912
• Protein Accession #: NP_001774.1; NP_067612.1
• Nucleotide Accession #: NM_001783.3; NM_021601.3