CD79A Antibody (OAEE00966)

Catalog No: OAEE00966

Size:100T

Surface staining of CD79a in human peripheral blood with anti-CD79a (HM47) APC.

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Dog|Guinea Pig|Horse|Human|Mouse|Non-human Primate|Porcine|Rabbit|Rat
• Product Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: HM47
• Isotype: IgG1
• Host: Mouse
• Conjugation: APC
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
• Purification: The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Specificity: The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
• Formulation: APC
• Application Info: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
• Reference: Islas-Ohlmayer M, Padgett-Thomas A, Domiati-Saad R, Melkus MW, Cravens PD, Martin Mdel P, Netto G, Garcia JV: Experimental infection of NOD/SCID mice reconstituted with human CD34+ cells with Epstein-Barr virus. J Virol. 2004 Dec;78(24):13891-900.
  Zhao XF, Hassan A, Perry A, Ning Y, Stass SA, Dehner LP: C-MYC rearrangements are frequent in aggressive mature B-Cell lymphoma with atypical morphology. Int J Clin Exp Pathol. 2008 Jan 1;1(1):65-74.
  Torlakovic E, Torlakovic G: B-cell markers in lymphocyte predominance Hodgkin disease. Arch Pathol Lab Med. 2002 Jul;126(7):862-3.
  Fernandez NJ, West KH, Jackson ML, Kidney BA: Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors. Vet Pathol. 2005 Jul;42(4):437-45.
  Rassenti LZ, Kipps TJ: Expression of Ig-beta (CD79b) by chronic lymphocytic leukemia B cells that lack immunoglobulin heavy-chain allelic exclusion. Blood. 2000 Apr 15;95(8):2725-7.
  Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147(11):2474-82.
  Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A: CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone-dependent manner. Am J Clin Pathol. 2007 Aug;128(2):306-13.
  

Target Info

• Gene Symbol: CD79A
• Gene Full Name: CD79a molecule
• Alias Symbols: B-cell antigen receptor complex-associated protein alpha chain;CD79a antigen (immunoglobulin-associated alpha);CD79a molecule, immunoglobulin-associated alpha;IGA;ig-alpha;MB-1;MB-1 membrane glycoprotein;membrane-bound immunoglobulin-associated protein;surface IgM-associated protein.
• NCBI Gene Id: 973
• Protein Name: B-cell antigen receptor complex-associated protein alpha chain
• Description of Target: Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.
• Uniprot ID: P11912
• Protein Accession #: NP_001774.1; NP_067612.1
• Nucleotide Accession #: NM_001783.3; NM_021601.3