CD27 Antibody : FITC (OATA00207)

Catalog No: OATA00207

Human peripheral blood lymphocytes were stained with 5 uL (1 ug) FITC Anti-Human CD27 (OATA00207) (solid line) or 1 ug FITC Mouse IgG1 isotype control.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Baboon, Cynomolgus, Rhesus
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: O323
• Isotype: Mouse IgG1, kappa
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: FC
  Excitation Laser: Blue (488 nm)
  Emmision: 520 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD27
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Reference:

  Kroenke MA, Eto D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow cytometry)

  Ruffell B, Au A, Rugo HS, Esserman LJ, Hwang ES, and Coussens LM. 2012. Proc. Natl. Acad. Sci. 109: 2796-2801. (Flow cytometry)

  Strowig T, Tongvaux A, Rathinam C, Takizawa H, Borsotti C, Philbrick W, Eynon EE, Manz, MG, and Flavell RA. 2011. Proc. Natl. Acad. Sci. 108: 13218-13223. (Flow cytometry)

  So NSY, Ostrowski MA and Gray-Owen SD. 2012. J. Immunol. 188: 4008-4022. (Flow cytometry)

  Klatt NR, Vinton CL, Lynch RM, Canary LA, Ho J, Darrah PA, Estes JD, Seder RA, Moir SL, and Brenchley JM. 2011. Blood. 118: 5803-5812. (Cell sorting - Rhesus)

  Ali Z, Yan L, Plagman N, Reichenberg A, Hintz M, Jomaa H, Villinger F, and Chen ZW. 2009. J. Immunol. 183: 5407-5417. (Flow cytometry - Rhesus)

  Ali Z, Shao L, Halliday L, Reichenberg A, Hintz M, Jomaa H, and Chen ZW. 2007. J. Immunol. 8287-8296. (Flow cytometry - Cynomolgus)

Target Info

• Gene Symbol: CD27
• Gene Full Name: CD27 molecule
• Alias Symbols: T14, S152, Tp55, TNFRSF7, S152. LPFS2
• NCBI Gene Id: 939
• Protein Name: CD27 antigen
• Description of Target: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
• Uniprot ID: P26842
• Protein Accession #: NP_001233.1
• Nucleotide Accession #: NM_001242.4