BK Beta3a Antibody (OASE00178)

Catalog No: OASE00178 (Formerly GWB-F12280)

Size:100ug

Western Blot analysis of Human Cell line lysates showing detection of BK Beta2 Potassium Channel protein using Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody, Clone S53-32. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-BK Beta2 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: S40B-18
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, IP, AM
• Additional Information: Background Info: Detects ~32 kDa. No cross-reactivity against BKBeta1, BKBeta1, BKBeta3b or BKBeta4.
  Scientific Background: BK channels contribute to electrical impulses, proper signal transmission of information and regulation of
  neurotransmitter release (3). A gain of function mutation in
  the pore-forming alpha subunit of the BK channel was linked
  to human neurological diseases. Findings suggest that the
  distribution of the beta subunits in the brain can modulate
  the BK channels to contribute to the pathophysiology of
  epilepsy and dyskinesia (4). This has major implications on
  other physiological processes in tissues other than the brain.
  Certificate of Analysis: 1 ug/mL was sufficient for detection of BK Beta3a in 10ug of COS cell (lysate) transiently transfected with BKbeta3a by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 1-49 (unique N-terminus) of mouse BKBeta3a
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~32 kDa. No cross-reactivity against BKBeta1, BKBeta2, BKBeta3b or BKBeta4.
• Dilution: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:
  Sunderland, MA USA.
  2. www.iochannels.org
  3. Wulf-Johansson H., et al. (2009) Brain Res. 1292: 1-
  13.
  4. Lee U.S., and Cui J. (2009) J Physiol. 587(7): 1481-
  1489.
• Description: BK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release (1). A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia (2). This has major implications on other physiological processes in tissues other than the brain.

Target Info

• Gene Symbol: KCNMB3
• Gene Full Name: potassium large conductance calcium-activated channel, subfamily M beta member 3
• Alias Symbols: HBETA3, KCNMB2, KCNMBL, BKBeta3, KCNMB3, Calcium-activated potassium channel subunit beta-3, BK channel subunit beta-3, BKbeta3, Hbeta3, Calcium-activated potassium channel, subfamily M subunit beta-3 Charybdotoxin receptor subunit beta-3, K(VCA)beta-3, Maxi K channel subunit beta-3, Slo-beta-3
• NCBI Gene Id: 27094
• Uniprot ID: Q9NPA1
• Protein Accession #: NP_055222.3