beta2-microglobulin Antibody (OAEE00027)

Catalog No: OAEE00027

Size:100UG

Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human beta2-microglobulin (B2M-01).
Lane 1: RAJI human Burkitt lymphoma cell line
Lane 2: EL4 mouse lymphoblastic lymphoma cell line
Lane 3: U937 human histiocytic lymphoma cell line

Confocal microscopy of human beta2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor 647 conjugate and B) with Alexa Fluor 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: B2M-01
• Isotype: IgG2a
• Host: Mouse
• Conjugation: Biotin
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Purified human beta2-microglobulin
• Purification: Purified by size-exclusion chromatography
• Concentration: 1 mg/ml
• Specificity: The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
• Formulation: Biotin
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Poláková K, Železníková T, Russ G: HLA-G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb;37(2):139-45.
  Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA-G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79(24): 15226-15237.
  Hilgert I, Horejsi V, Kristofova H: The use of murine monoclonal antibody B2M-01 for detection and purification ofhuman beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
  Khurana M, Traum AZ, Aivado M, Wells MP, Guerrero M, Grall F, Libermann TA, Schachter AD: Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol. 2006 Sep;21(9):1257-65. Epub 2006 Jun 30.
  

Target Info

• Gene Symbol: B2M
• Gene Full Name: beta-2-microglobulin
• Alias Symbols: beta chain of MHC class I molecules;beta-2-microglobin;beta-2-microglobulin;IMD43.
• NCBI Gene Id: 567
• Protein Name: Beta-2-microglobulin
• Description of Target: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
• Uniprot ID: P61769
• Protein Accession #: NP_004039.1
• Nucleotide Accession #: NM_004048.2