AXL Antibody (OAAB19577)

Catalog No: OAAB19577

Size:100ul

Western blot analysis of lysate from Hela cell line, using Rabbit Anti-AXL antibody (OAAB19577). OAAB19577 was diluted at 1: 1000. A goat anti-rabbit IgG H&L (HRP) at 1: 10000 dilution was used as the secondary antibody.Lysate at 20ug.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Rabbit, Dog, Horse
• Product Format: Liquid. 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC-P
  Cellular Location: Cell membrane; Single-pass type I membrane protein.
  Tissue Location: Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Intended Use: For research use only.
• Application Info: IHC-P: 1:100~1:500
  WB: 1:1000
• Reference: Partanen J.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:8913-8917(1990).
  O'Bryan J.P.,et al.Mol. Cell. Biol. 11:5016-5031(1991).
  Janssen J.W.G.,et al.Oncogene 6:2113-2120(1991).
  Grimwood J.,et al.Nature 428:529-535(2004).
  Lee S.-T.,et al.Oncogene 8:3403-3410(1993).
  

Target Info

• Gene Symbol: AXL
• Alias Symbols: ARK, UFO, JTK11, Tyro7
• NCBI Gene Id: 558
• Description of Target: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.
• Uniprot ID: P30530
• Molecular Weight: 98 kDa