AXL Antibody (Phospho-Tyr691) (OAAF00433)

Catalog No: OAAF00433 (Formerly GWB-ASB877)

Size:100 ug

Western blot analysis of extracts from HuvEc cells treated with EGF 200ng/ml 15', using AXL (Phospho-Tyr691) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using AXL (Phospho-Tyr691) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
  Target Modification: Phospho
  Modification Sites: H:Y691, M:Y692, R:Y692
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human AXL around the phosphorylation site of Tyr691.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Specificity: AXL (Phospho-Tyr691) antibody detects endogenous levels of AXL only when phosphorylated at tyrosine 691.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1000
  IHC: 1:50~100
  ELISA: 1:20000
• Reference: Partanen J., Proc. Natl. Acad. Sci. U.S.A. 87:8913-8917(1990).
  Janssen J.W.G., Oncogene 6:2113-2120(1991).
  O'Bryan J.P., Mol. Cell. Biol. 11:5016-5031(1991).

Target Info

• Gene Symbol: AXL
• Gene Full Name: AXL receptor tyrosine kinase
• Alias Symbols: ARK;AXL oncogene;AXL transforming sequence/gene;JTK11;Tyro7;tyrosine-protein kinase receptor UFO;UFO.
• NCBI Gene Id: 558
• Protein Name: Tyrosine-protein kinase receptor UFO
• Description of Target: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.
• Uniprot ID: P30530
• Protein Accession #: NP_001690.2
• Molecular Weight: 97 kDa