AXL Antibody - C-terminal region (OAAB17122)

Catalog No: OAAB17122

Size:400ul

Western blot analysis of anti-AXL C-term Pab (Cat. # OAAB17122) in SKBR3 cell lysate. AXL (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, ELISA
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein
  Tissue Location: Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This AXL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 863-894 amino acids from the C-terminal region of human AXL.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 0.5 mg/ml
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
• Publications:

  Chemotherapy confers a conserved secondary tolerance to EGFR inhibition via AXL-mediated signaling bypass. Sci Rep. 11, 8016 (2021). 33850249

Target Info

• Gene Symbol: AXL
• Alias Symbols: ARK, UFO, JTK11, Tyro7
• NCBI Gene Id: 558
• Protein Name: Tyrosine-protein kinase receptor UFO
• Description of Target: AXL, a member of the AXL/UFO subfamily of Tyr protein kinases, may function as a signal transducer between specific cell types of mesodermal origin. This Type I membrane protein has transforming potential in patients with chronic myeloproliferative disorder or chronic myelocytic leukemia. The protein contains 2 putative fibronectin type III domains and 2 putative immunoglobulin-like C2-type domains.
• Uniprot ID: P30530
• Protein Accession #: NP_001690.2
• Molecular Weight: 98 kDa