AXL Antibody (OAAD00304)

Catalog No: OAAD00304

Size:0.1ml

western blot analysis using AXL mouse mAb against extracellular domain of human AXL (aa19-444).

Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with AXL-hIgGFc using AXL mouse mAb(green), showing cytoplasmic and membrane localization. Blue: DRAQ5 fluorescent DNA dye.

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: 7E10
• Isotype: IgG1
• Host: Mouse
• Application: WB, IF
• Reconstitution and Storage: Store at 4C, for long term storage, store at -20C.
• Immunogen: Purified recombinant extracellular fragment of human AXL fused with hIgGFc tag expressed in HEK293 cell line.
• Formulation: Ascitic fluid containing 0.03% sodium azide.
• Application Info: Western Bloting: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test.
• Reference: 1. Br J Cancer. 2006 May 22;94(10):1446-51. 2. Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5799-804.

Target Info

• Alias Symbols: ARK, UFO, JTK11, Tyro7
• NCBI Gene Id: 558
• Description of Target: AXL: AXL receptor tyrosine kinase, also known as UFO, JTK11. Entrez Protein NP_001690. It is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Uniprot ID: P30530
• Molecular Weight: 97.4kDa