ATF4 ELISA Kit (Rat) (OKCD01199)

Catalog No: OKCD01199 (Formerly GWB-KBBLN1)

Size:96WELLS

Rat Atf4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	78-101%	90-97%	78-101%	84-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Transcription Factor 4 (ATF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activating Transcription Factor 4 (ATF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Transcription Factor 4 (ATF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Activating Transcription Factor 4 (ATF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	80-97	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activating Transcription Factor 4 (ATF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activating Transcription Factor 4 (ATF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Atf4 Microplate: 96 Wells (12 x 8 Well Strips)
  • Atf4 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Atf4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 28 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Activating Transcription Factor 4 (ATF4). No significant cross-reactivity or interference between Activating Transcription Factor 4 (ATF4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Atf4
• Gene Full Name: activating transcription factor 4
• Alias Symbols: Activating transcription factor 4;activating transcription factor 4 (tax-responsive enhancer element B67);activating transcription factor ATF-4;cAMP-dependent transcription factor ATF-4;cyclic AMP-dependent transcription factor ATF-4;rATF-4.
• NCBI Gene Id: 79255
• Protein Name: Cyclic AMP-dependent transcription factor ATF-4
• Description of Target: Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to ER stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes (By similarity).
• Uniprot ID: Q9ES19
• Protein Accession #: NP_077379
• Nucleotide Accession #: NM_024403.2
• Protein Size (# AA): 347