Anti-CD68/Macrophage Marker Antibody (OAAI00274)

Catalog No: OAAI00274

Size:100UG

IHC: Human Tonsil tissue stained with Anti-CD68 antibody, (Cat#OAAI00274 ) at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Monoclonal
• Clone: KP1
• Isotype: IgG1
• Host: Mouse
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: CD68 is an intracellular glycoprotein expressed on macrophages and monocytes. CD68 expression has been demonstrated in T cells, NK cells, liver and renal tubules. This antibody is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.
  Positive Control: Tonsil, Lymph Node, Spleen
  Cellular Location: Cytoplasmic /Membrane
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: Subcellular fraction of human alveolar macrophages
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Concentration: 100ug/200ul
• Specificity: CD68
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: De Groot CJ; et al. Journal of Neuropathology and Experimental Neurology, 1997 Jan, 56(1):10-20.

Target Info

• Gene Symbol: CD68
• Gene Full Name: CD68 molecule
• Alias Symbols: CD68 antigen;GP110;LAMP4;macrophage antigen CD68;macrosialin;SCARD1;scavenger receptor class D, member 1.
• NCBI Gene Id: 968
• Description of Target: CD68 is an intracellular glycoprotein expressed on macrophages and monocytes. CD68 expression has been demonstRated in T cells, NK cells, liver and renal tubules. This Antibody is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.
• Uniprot ID: P34810
• Molecular Weight: 110