SAVE NOW with 10% off all Recombinant Antibodies Shop Now

Catalog No: OASA04073 (Formerly GWB-Q01303)
Size:100UG
Price: $419.00
SKU
OASA04073
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for Cd68 Antibody (OASA04073)
Product Info
Predicted Species ReactivityRat
Product FormatLiquid. Phosphate buffered saline
ClonalityMonoclonal
CloneED1
IsotypeIgG1
HostMouse
ApplicationFC, IF, IHC-P, WB
Additional InformationFusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
::Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
::Approx Protein Conc: IgG concentration 0.5 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Reconstitution and Storage-20°C
ImmunogenRat spleen cells
PurificationProtein A Purified
Predicted Homology Based on Immunogen SequenceRat
Concentration0.5 mg/ml
SpecificityCD68
Application InfoFlow Cytometry(1): 1/50 - 1/100
Application DataApplication #1: Staining of rat liver, with induced hepatocellular damage, with Mouse anti Rat CD68
Application #2: Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE . Permeabilised with Leucoperm (Fix & Perm) (BUF09)
Application #3: Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:FITC following permeabilisation with Leucoperm (BUF09)
Application #4: Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm (BUF09)
Application #5: Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 488 . following permeabilisation with Leucoperm (BUF09)
Protocol InformationCitation: 1: Campbell SJ, Perry VH, Pitossi FJ, Butchart AG, Chertoff M, Waters S, DempsterR, Anthony DC. Central nervous system injury triggers hepatic CC and CXCchemokine expression that is associated with leukocyte mobilization andrecruitment to both the central nervous system and the liver. Am J Pathol. 2005May;166(5):1487-97. PubMed PMID: 15855648; PubMed Central PMCID: PMC1606402.
Species: Rat
Experiment Name: 1. Identification of Leukocytes2. Immunocytochemistry for CCL-2
Experiment Background: The administration of interleukin-1β to the brain induces hepatic CXC chemokine synthesis, which increases neutrophil levels in the blood, liver, and brain. Sandra et al. showed that such hepatic response is not restricted to the CXC chemokines. CCL-2, a CC chemokine, was released by the liver in response to a tumor necrosis factor (TNF)-α challenge to the brain and boosted monocyte levels. Furthermore, a clinically relevant compression injury to the spinal cord triggered hepatic chemokine expression of both types.
Experimental Steps: 1. Identification of LeukocytesFrozen, 10-um-thick serial coronal sections were cut from tissue blocks, through the injection site in the brain, through a representative lobe of liver or in 20-um-thick parasagittal sections through the compression-injured spinal cord. Using immunohistochemistry, neutrophils were identified using the anti-neutrophil serum HB199, activated macrophages/Kupffer cells and recruited monocytes were identified using the anti-ED-1 serum (Oxford, UK). For each tissue section, four representative fields were chosen for quantitation and the average number of positive cells was calculated and expressed as number of cells per mm2. Tissue sections were counterstained with cresyl-violet and immunopositive labeling was quantitated only when associated with a cell nucleus.2. Immunocytochemistry for CCL-2Before immunohistochemistry, antigens were retrieved from Bouin’s-fixed tissue by microwaving for 11 minutes at 650 W. Ten-um paraffin wax sections were stained with anti-rat CCL-2 goat polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA) using standard methodology. In double-labeling studies, sections were initially stained for CCL-2, which was revealed using diaminobenzidine as the chromogen (brown precipitate), before using cell identification markers for ED-1 which was revealed by very intense purple (VIP) (Vector Laboratories, Peterborough, UK) (purple precipitate). Amendments were required for the identification of CCL-2 in liver. Formalin-fixed liver sections were dewaxed and rehydrated using standard procedures. Antigens for the antibodies ED-1 and HB19926 were revealed using a pressure cooker (121C for 20 minutes). Sections were incubated for 1 hour in the respective antibodies according to the manufacturers’ instructions. Labeling for cell markers was revealed using directly horseradish peroxidase-conjugated secondary antibodies to mouse or rabbit IgG (Sigma-Aldrich) to eliminate labeling of endogenous hepatic biotin. Retrieval of CCL-2 antigens in the liver required an additional pretreatment (0.04% pepsin in 0.1% HCl for 20 minutes) before overnight incubation at 4C in anti-CCL-2 rabbit polyclonal antibody (AbCam Ltd., Cambridge, UK). CCL-2 antibody was detected using biotinylated anti-rabbit IgG and standard ABC (Vector Laboratories) because amplification was necessary to reveal CCL-2. Single-labeled and double-labeled immunohistochemistry in the liver was revealed using diaminobenzidine and VIP as described above.
Other Reagents Used: Dexamethasone 21-phosphate, EDTA
Number Of Protocols: 2
Reference1. Damoiseaux, J.G. et al. (1994) Rat macrophage lysosomal membrane antigen recognised by monoclonal antibody ED1. Immunol. 83: 140-147.
2. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognised by monoclonal antibodies ED1, ED2 and ED3. Immunol 54: 589-599.
3. Bauer, J. et al. (1994) Phagocytic activity of macrophages and microglial cells during the course of Acute and Chronic Relapsing Experimental Autoimmune Encephalomyelitis. J. Neurosci. Res. 38: 365-375.
4. Bao, F. et al. (2004) Early anti-inflammatory treatment reduces lipid peroxidation and protein nitration after spinal cord injury in rats. J, Neuro-chem. 88:1335-1344.
5. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and
Gene SymbolCd68
Alias SymbolsMGC114377, Cd68
NCBI Gene Id287435
Protein NameCd68 molecule EMBL AAH98931.1
Description of TargetMOUSE ANTI RAT CD68
Uniprot IDQ4FZY1
Protein Accession #NP_001026808.1
Protein Size (# AA)330
  1. What is the species homology for "Cd68 Antibody (OASA04073)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Rat".

  2. How long will it take to receive "Cd68 Antibody (OASA04073)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Cd68 Antibody (OASA04073)" provided in?

    This item is provided in "Liquid. Phosphate buffered saline".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Cd68 Antibody (OASA04073)"?

    This target may also be called "MGC114377, Cd68" in publications.

  5. What is the shipping cost for "Cd68 Antibody (OASA04073)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Cd68 Antibody (OASA04073)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Cd68 Antibody (OASA04073)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Cd68 Antibody (OASA04073)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "CD68"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CD68"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CD68"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CD68"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CD68"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CD68"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

Write Your Own Review
You're reviewing:Cd68 Antibody (OASA04073)
Your Rating
We found other products you might like!