Cd68 Antibody (OASA04074)

Catalog No: OASA04074 (Formerly GWB-Q01304)

Size:250UG

Staining of rat liver, with induced hepatocellular damage, with Mouse anti Rat CD68

Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE. Permeabilised with Leucoperm (Fix & Perm) (BUF09)

Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:FITC following permeabilisation with Leucoperm (BUF09)

Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm (BUF09)

Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 488. following permeabilisation with Leucoperm (BUF09)

Product Info

• Predicted Species Reactivity: Rat
• Product Format: Liquid. Phosphate buffered saline.
• Clonality: Monoclonal
• Clone: ED1
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Additional Information: Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
  Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  Preservative Stabilisers: 0.09% - Sodium Azide
  Approx Protein Conc: IgG concentration 0.5 mg/ml
  Buffer Solutions: Phosphate buffered saline pH7.4
• Reconstitution and Storage: -20°C
• Immunogen: Rat spleen cells
• Purification: Protein A Purified
• Predicted Homology Based on Immunogen Sequence: Rat
• Concentration: 1 mg/ml
• Specificity: CD68
• Application Info: Flow Cytometry(1): 1/50 - 1/100
  Immunohistology - Paraffin(2): 1/100
  
• Application Data: Application #1: Staining of rat liver, with induced hepatocellular damage, with Mouse anti Rat CD68
  Application #2: Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE . Permeabilised with Leucoperm (Fix & Perm) (BUF09)
  Application #3: Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:FITC following permeabilisation with Leucoperm (BUF09)
  Application #4: Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm (BUF09)
  Application #5: Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 488 . following permeabilisation with Leucoperm (BUF09)
• Protocol Information: Citation: 1: Huang W, Metlakunta A, Dedousis N, Zhang P, Sipula I, Dube JJ, Scott DK, O'Doherty RM. Depletion of liver Kupffer cells prevents the development of diet-induced hepatic steatosis and insulin resistance. Diabetes. 2010 Feb;59(2):347-57. Epub 2009 Nov 23. PubMed PMID: 19934001; PubMed Central PMCID: PMC2809951.
  Species: Rat
  Experiment Name: Immunohistochemistry and immunofluorescence.
  Experiment Background: In the current study, depletion of Kupffer cells was evaluated by quantification of CD68 (a macrophage marker)-positive cells by immunohistochemistry
  Experimental Steps: 1. For immunohistochemistry, tissues (liver and epididymal fat of Rat) were fixed overnight in a 4% paraformaldehyde solution, and subsequently embedded in paraffin.2. Sections were obtained and stained using standard techniques with CD68.CD68-positive cells were detected using α-CD68 (AbD ) and were quantified on 8–9 fields of ×40 sections using an Olympus (New York, NY) light microscope.3. For immunofluorescence, CD68 and TNFα were detected in liver sections after antigen retrieval by heating sections in 1.6 mmol/l EDTA in a high-pressure cooker for 7 min.4. Subsequently, sections were immunostained with α-CD68 (1:200) and α-TNFα (1:100; MBL, Woburn, MA) followed by incubation with Alexa 488–conjugated or cyanin 3–conjugated secondary antibodies, respectively. Images were visualized using an Olympus Provis fluorescence microscope and modified with Magnafire software (Olympus, Melville, NY).
  Other Reagents Used: Gadolinium chloride, NaH2PO4, FBS, glucose.
  Number Of Protocols: 2
• Reference: 1. Damoiseaux, J.G. et al. (1994) Rat macrophage lysosomal membrane antigen recognised by monoclonal antibody ED1. Immunol. 83: 140-147.
  2. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognised by monoclonal antibodies ED1, ED2 and ED3. Immunol 54: 589-599.
  3. Bauer, J. et al. (1994) Phagocytic activity of macrophages and microglial cells during the course of Acute and Chronic Relapsing Experimental Autoimmune Encephalomyelitis. J. Neurosci. Res. 38: 365-375.
  4. Bao, F. et al. (2004) Early anti-inflammatory treatment reduces lipid peroxidation and protein nitration after spinal cord injury in rats. J, Neuro-chem. 88:1335-1344.
  5. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and

Target Info

• Gene Symbol: Cd68
• Alias Symbols: MGC114377, Cd68
• NCBI Gene Id: 287435
• Protein Name: Cd68 molecule EMBL AAH98931.1
• Description of Target: MOUSE ANTI RAT CD68
  
• Uniprot ID: Q4FZY1
• Protein Accession #: NP_001026808.1
• Protein Size (# AA): 330