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Catalog No: OASA05367 (Formerly GWB-Q01476)
Size:250UG
Price: $499.00
SKU
OASA05367
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for Cd68 Antibody (OASA05367)
Product Info
Predicted Species ReactivityMouse
Product FormatLiquid. Phosphate buffered saline
ClonalityMonoclonal
CloneFA-11
IsotypeIgG2a
HostRat
ApplicationFC, IF, IHC-P, WB
::Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
::Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Reconstitution and Storage-20°C
ImmunogenPurified Concanavalin A acceptor glycoprotein from P815 cell line.
PurificationProtein G Purified
Predicted Homology Based on Immunogen SequenceMouse
Concentration1 mg/ml
SpecificityCD68
Application InfoFlow Cytometry(1): 1/50 - 1/100
Application DataApplication #1: Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:Biotin .following permeablisation with Leucoperm (BUF09)
Application #2: Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:FITC . following permeablisation with Leucoperm (BUF09)
Application #3: Staining of permeabilised mouse peritoneal macrophages cells with Rat anti Mouse CD68 visualised with F(ab')2 Goat anti Rat IgG:FITC (Mouse adsorbed)
Application #4: Staining of mouse peritoneal macrophages with Rat anti Mouse CD68 CD68 , following permeablisation with Leucoperm (BUF09)
Application #5: Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm (BUF09)
Protocol InformationCitation: 1: Sun Y, Liou B, Ran H, Skelton MR, Williams MT, Vorhees CV, Kitatani K, Hannun YA, Witte DP, Xu YH, Grabowski GA. Neuronopathic Gaucher disease in the mouse: viable combined selective saposin C deficiency and mutant glucocerebrosidase (V394L) mice with glucosylsphingosine and glucosylceramide accumulation and progressive neurological deficits. Hum Mol Genet. 2010 Mar 15;19(6):1088-97. Epub 2010 Jan 4. PubMed PMID: 20047948; PubMed Central PMCID: PMC2830832.
Species: Mouse
Experiment Name: Immunohistochemistry
Experiment Background: Axonal degeneration was evident in brain stem, spinal cord and white matter of cerebellum accompanied by increasing infiltration of the brain stem, cortex and thalamus by CD68 positive microglial cells and activation of astrocytes.Here CD68 positive staining demonstrated activation of macrophage/ microglial cells in 4L;C* mice brain stem, spinal cord, and thalamus.
Experimental Steps: 1.The 4L;C* and strain-matched WT mice were subjected to CO2 narcosis and then, perfused with saline followed by 4% paraformaldehyde before tissue dissection.2. The tissues were fixed in 10% formalin or 4% paraformaldehyde and processed for paraffin or frozen blocks, respectively.3. Paraffin sections of brain were stained with H&E. Karnovsky's fixative was used for ultrastructural studies. CD68 monoclonal antibody staining was perfomed.4. Frozen sections were stained with mouse anti-pig glial fibrillary acidic protein (GFAP). Biotinylated goat anti-mouse followed by streptavidin-conjugated fluorescent 488 (green) and goat anti-rat conjugated to FITC were applied to the sections.5. Both mouse anti-pig GFAP and mouse anti-recombinant p62 antibodies have reactivities in the mouse tissues.6. Colocalization of p62 in neuron, astrocyte or microglial cells was carried out on frozen sections using mouse anti-recombinant p62 (1:100) followed by biotinylated goat anti-mouse and streptavidin-conjugated fluorescent 610 (red).]7. Mouse anti-NeuN conjugated to Alexa Fluor 488 (1:100), mouse anti-GFAP conjugated to Alexa Fluor 488 (1:100) or rat anti-CD68 (1:100) were applied as marker for neuron, astrocyte or microglial cells, respectively. Rat anti-CD68 was detected by biotinylated goat anti-rat and streptavidin-conjugated fluorescent 488 (green). DAPI (blue) in anti-fade were used for cell nuclei staining.
Number Of Protocols: 1
Reference1. Ramprasad, M.P. et al. (1996) Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein. Proc. Natl. Acad. Sci. 93: 14833-14838.
2. Rabinowitz, S.S. & Gordon, S. (1991) Macrosialin, a macrophage-restricted membrane sialoprotein differentially glycosylated in response to inflammatory stimuli. J. Exp. Med. 174: 827-836.
3. Da Silva, R.P & Gordon, S. (1999) Phagocytosis stimulates alternative glycosylation of macrosialin (mouse CD68), a macrophage-specific endosomal protein. Biochem. J. 338: 687-694.
4. Schleicher, U. et al. (2005) Minute numbers of contaminant CD8+ T cells or CD11b+CD11c+ NK cells are the source of IFN-{gamma} in IL-12/IL-18-stimulated mouse macrophage populations. Blood 105: 1319-1328.
5. von Lukowicz, T. et al. (2008) PARP1 is required for adhesion molecule expression in atherogenesis. Cardiovasc. Res. 78: 158-166
Gene SymbolCd68
Alias SymbolsLa, ma, Sca, gp11, Lamp4, gp110, Scard1
NCBI Gene Id12514
Protein NameMacrosialin
Description of TargetRAT ANTI MOUSE CD68
Uniprot IDP31996
Protein Accession #NP_033983.1
Protein Size (# AA)335
  1. What is the species homology for "Cd68 Antibody (OASA05367)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse".

  2. How long will it take to receive "Cd68 Antibody (OASA05367)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Cd68 Antibody (OASA05367)" provided in?

    This item is provided in "Liquid. Phosphate buffered saline".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Cd68 Antibody (OASA05367)"?

    This target may also be called "La, ma, Sca, gp11, Lamp4, gp110, Scard1" in publications.

  5. What is the shipping cost for "Cd68 Antibody (OASA05367)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Cd68 Antibody (OASA05367)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Cd68 Antibody (OASA05367)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Cd68 Antibody (OASA05367)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "CD68"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CD68"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CD68"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CD68"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CD68"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CD68"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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