APRIL Antibody (OAED00315)

Catalog No: OAED00315

Size:100UG

Figure 1: Western blot of total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL. Method: 10 ug of protein was applied to the gel. Revealed with Aprily-8 (1 ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4’000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).

Figure 2: FACS analysis of cells with MAb to APRIL (Aprily-8). Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x105) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10 ug/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.

Figure 3: Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with 4% formaldehyde 5 min. at RT. After a wash in PBS, samples were dehydrated by washes in 60%, 80%, 90%, 100% EtOH and xylol. Cells were then dried and embedded in paraffin. Sections were cut, mounted on slides and dried overnight at 50C. Slides were then successively washed 2x 10 min. in xylol, 2x 10 min. in 100% ethanol, and then treated 10 min. in 100% methanol/0.6% H202 to inhibit endogenous peroxidase. Samples were rehydrated by washes in 90%, 80%, 60% ethanol and PBS. After micro-wave treatment, slides were washed 3x in PBS, blocked with IgG, and incubated for 1 hour with 5 ug/ml Aprily-8 or control mouse IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After PBS washes, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS containing 0.02% sodium azide.
• Clonality: Monoclonal
• Clone: Aprily-8
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunocytochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: Store short-term at 4°C and long-term at -20°C. Avoid freeze/thaw cycles. Stable for one year after receipt when stored at -20°C.
• Immunogen: Recombinant human APRIL (aa 93-233).
• Concentration: 1 mg/ml
• Specificity: Recognizes human APRIL and TWE-PRIL. Does not cross-react with mouse APRIL and TWE-PRIL.
• Source: Purified from concentrated hybridoma tissue supernatant.
• Application Info: Excellent for Immunocytochemistry and Western Blot.
• Reference: Expression of TNF-superfamily members BAFF and APRIL in breast cancer: Immunohistochemical study in 52 invasive ductal breast carcinomas: V. Pelekanou, et al.; BMC Cancer 8, 76 (2008), Abstract; Full Text
  BAFF and APRIL: A Tutorial on B Cell Survival: F. Mackay, et al.; Annu. Rev. Immunol. (2002), Abstract;

Target Info

• Gene Symbol: TNFSF13
• Gene Full Name: TNF superfamily member 13
• Alias Symbols: a proliferation-inducing ligand;APRIL;CD256;TALL2;TALL-2;TNF- and APOL-related leukocyte expressed ligand 2;TNF-related death ligand 1;TNLG7B;TRDL-1;tumor necrosis factor (ligand) superfamily, member 13;tumor necrosis factor ligand 7B;tumor necrosis factor ligand superfamily member 13;tumor necrosis factor superfamily member 13;tumor necrosis factor-like protein ZTNF2;tumor necrosis factor-related death ligand-1;UNQ383/PRO715;ZTNF2.
• NCBI Gene Id: 8741
• Protein Name: Tumor necrosis factor ligand superfamily member 13
• Description of Target: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
• Uniprot ID: O75888