AMH Antibody - middle region (OAAB06540)

Catalog No: OAAB06540

Size:200ul

Western blot analysis of AMH Antibody (Center) in 293 cell lysates (35ug/lane). AMH (arrow) was detected using the purified Pab.

AMH is strongly supported by BioGPS gene expression data to be expressed in HEK293

Western blot analysis of AMH Antibody (Center) in mouse liver tissue lysates (35ug/lane). AMH (arrow) was detected using the purified Pab.

AMH Antibody (Center) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, FC, ELISA
• Additional Information: Cellular Location: Secreted.
  Tissue Location: In ovaries, AMH is detected in granulosa cells of early growing follicles.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This AMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-451 amino acids of human AMH.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.5 mg/ml
• Sample Type Confirmation:

  AMH is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: WB: 1:1000~2000
  IHC-P: 1:25
  FC: 1:25
• Publications:

  Structure of AMH bound to AMHR2 provides insight into a unique signaling pair in the TGF-β family. Proc Natl Acad Sci U S A. 118 (2021) 34155118

Target Info

• Gene Symbol: AMH
• Alias Symbols: MIF, MIS
• NCBI Gene Id: 268
• Protein Name: Muellerian-inhibiting factor
• Description of Target: Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
• Uniprot ID: P03971
• Protein Accession #: NP_000470.2
• Molecular Weight: 59 kDa