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Catalog No: OKEH00319 (Formerly GWB-EOW5R3)
Size:96T
Price: $425.00
SKU
OKEH00319
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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AMH ELISA Kit (Human) : 96 Wells (OKEH00319)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00319
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AMH ELISA Kit (Human) (OKEH00319) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AMH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for AMH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AMH captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
ComponentAmount
AMH Microplate96 Wells (12 x 8 Well strips)
AMH Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated AMH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.45 ng/mL
SpecificityNatural and recombinant Human Muellerian-inhibiting factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAMH
Gene Full Nameanti-Mullerian hormone
Alias Symbolsanti-Muellerian hormone;MIF;MIS;muellerian-inhibiting factor;muellerian-inhibiting substance;Mullerian inhibiting factor;Mullerian inhibiting substance.
NCBI Gene Id268
Protein NameP03971
Description of TargetMuellerian-inhibiting factor
Uniprot IDhttps://www.uniprot.org/uniprot/P03971
Protein Accession #NP_000470.2
Nucleotide Accession #NM_000479.3
Protein Size (# AA)560
Molecular Weight59 kDa
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