Aha1 Antibody (OASE00108)

Catalog No: OASE00108 (Formerly GWB-225226)

Size:25ug

Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).

Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: 25F2.D9
• Isotype: IgG2a Kappa
• Host: Rat
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, IP, ELISA
• Additional Information: Background Info: ~38kDa. Can run up to 45kDa on SDS Page
  Scientific Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Aha1 in 10ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Recombinant Full Length Mouse Aha1 Protein
• Purification: Protein G Purified
• Concentration: 1.2 mg/ml
• Specificity: Detects ~38kDa. Can run up to 45kDa on SDS Page.
• Dilution: WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user.
• Storage Buffer: 0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
• Reference: 1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub.
  2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796.
  3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723.
  4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.
  
• Description: Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Target Info

• Gene Symbol: AHA1
• Gene Full Name: AHA1, activator of heat shock protein ATPase 1
• Alias Symbols: Aha 1, Ahsa1, p38, HSPC322, C14orf3, Activator of HSP90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1
• NCBI Gene Id: 217737
• Uniprot ID: Q8BK64
• Protein Accession #: NP_666148.1