Size:96 Wells
$599.00
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SKU
OKCD04383
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ABCC8 ELISA Kit (Human) (OKCD04383)

Catalog#: OKCD04383
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology ABCC8 ELISA Kit (Human) (OKCD04383) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCC8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCC8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCC8 captured in the well.
Kit Range 1.25-20 ng/mL
Kit Reproducibility Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level ABCC8. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level ABCC8 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Component
ComponentAmount
ABCC8 Microplate96 Wells (12 x 8 Well strips)
ABCC8 Lyophilized Standard2
100X ABCC8 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Tissue homogenates, cell lysates and other biological fluids
Sensitivity 0.52 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol ABCC8
Alias Symbols HI, SUR, HHF1, MRP8, PHHI, SUR1, ABC36, HRINS, TNDM2, SUR1delta2
NCBI Gene Id 6833
Protein Name ATP-binding cassette sub-family C member 8
Description of Target The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternatively spliced transcript variants have been found for this gene.
Swissprot Id Q09428
Protein Accession # NP_000343.2
Nucleotide Accession # NM_000352.4
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ABCC8.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ABCC8.
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