Goat Anti-SUR1 / ABCC8 Antibody (OAEB02180)

Catalog No: OAEB02180 (Formerly GWB-EV0322)

Size:100ug

OAEB02180 (0.5ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Rabbit
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Pig: 92%; Rabbit: 92%; Rat: 92%
• Peptide Sequence: C-EFDKPEKLLSRKD
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Approx 170kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 177kDa according to NP_000343.2). Recommended concentration: 0.5-1.5ug/ml.
  An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunising peptide.
• Reference: * de Wet H, Rees MG, Shimomura K, Aittoniemi J, Patch AM, Flanagan SE, Ellard S, Hattersley AT, Sansom MS, Ashcroft FM.; Increased ATPase activity produced by mutations at arginine-1380 in nucleotide-binding domain 2 of ABCC8 causes neonatal diabetes.; Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18988-92.; Pubmed 18025464

Target Info

• Gene Symbol: ABCC8
• Alias Symbols: HI, SUR, HHF1, MRP8, PHHI, SUR1, ABC36, HRINS, PNDM3, TNDM2, SUR1delta2
• NCBI Gene Id: 6833
• Protein Name: ATP-binding cassette sub-family C member 8
• Uniprot ID: Q09428
• Protein Accession #: NP_000343.2