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- $55: Antibody & Protein in US
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| Datasheets/Manuals | Printable datasheet for 5-METHYLCYTIDINE Antibody (OASA00177) |
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| Predicted Species Reactivity | All Species |
|---|---|
| Product Format | Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | 33D3 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | Enzyme-linked immunosorbent assay|Flow cytometry|Hydroxymethylated DNA immunoprecipitation|Immunoblot|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Methylated DNA immunoprecipitation |
| Additional Information | Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag 14 myeloma cell line. |
| :: | Preservative Stabilisers: 0.01% - Thiomersal |
| :: | Approx Protein Conc: IgG concentration 1.0 mg/ml Buffer Solutions: Phosphate buffered saline pH7.4 |
| Reconstitution and Storage | -20°C |
| Purification | Protein A Purified |
| Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
| Concentration | 1 mg/ml |
| Specificity | OASA00177 recognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumors, DNA is frequently globally hypo-methylated compared to the DNA from normal tissue. Clone 33D3 has been developed to discriminate between the modified base 5-MeCyd and the normal counterpart cytosine. OASA00177 has been reported for use in Methylated DNA immunoprecipitation (MeDIP). |
| Reference | 1. Reynaud, C. et al. (1992) Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies. Cancer Lett. 61: 255-262. 2. Habib, M. et al. (1999) DNA Global Hypomethylation in EBV - Transformed Interphase Nuclei. Exp. Cell. Res. 249: 46-53. 3. Hernandez-Blazquez, F. et al. (2000) Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gut. 47: 689-693. 4. Giraldo, A. M. et al. (2007) DNA methylation and histone acetylation patterns in cultured bovine fibroblasts |
| Description of Target | MOUSE ANTI 5-METHYLCYTIDINE |
|---|
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "5-METHYLCYTIDINE Antibody (OASA00177)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "All Species".
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How long will it take to receive "5-METHYLCYTIDINE Antibody (OASA00177)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "5-METHYLCYTIDINE Antibody (OASA00177)" provided in?
This item is provided in "Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "5-METHYLCYTIDINE Antibody (OASA00177)"?
This target may also be called "" in publications.
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What is the shipping cost for "5-METHYLCYTIDINE Antibody (OASA00177)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "5-METHYLCYTIDINE Antibody (OASA00177)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "5-METHYLCYTIDINE Antibody (OASA00177)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "5-METHYLCYTIDINE Antibody (OASA00177)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with ""?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with ""?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with ""?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with ""?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
