WNT10A Antibody (OAGA00891)

Catalog No: OAGA00891

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
OAGA00891 diluted at 1:1000

WNT10A antibody [N1C1-2] detects WNT10A protein at cytoplasm by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WNT10A protein stained by WNT10A antibody [N1C1-2] (OAGA00891) diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, WB
• Additional Information: Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region. [provided by RefSeq]
  Positive Controls: Molt-4, Raji
  Cellular Localization: Secreted, extracellular space, extracellular matrix
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 47 and 271 of WNT10A (Uniprot ID#Q9GZT5)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: WNT10A
• Gene Full Name: Protein Wnt-10a
• Alias Symbols: OODD, SSPS, STHAG4
• NCBI Gene Id: 80326
• Protein Name: Protein Wnt-10a
• Uniprot ID: Q9GZT5
• Protein Accession #: NP_079492.2
• Molecular Weight: 46 kDa