Catalog No: OKEH03519 (Formerly GWB-GNLRNP)
Size:96T
Price: $525.00
SKU
OKEH03519
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology WNT10A ELISA Kit (Mouse) (OKEH03519) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Wnt10a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Wnt10a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Wnt10a captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.6% (n = 20)
Mean Inter-assay CV%: <= 6.4% (n = 20)
ELISA Kit Component
ComponentAmount
Wnt10a Microplate96 Wells (12 x 8 Well strips)
Wnt10a Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Wnt10a Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.083 ng/mL
SpecificityNatural and recombinant Mouse Protein Wnt-10a
Publications

Whitson JA et al. Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis. Invest Ophthalmol Vis Sci. 58, 2666 - 2684 (2017). 28525556

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolWnt10a
Gene Full Namewingless-type MMTV integration site family, member 10A
Alias Symbolsprotein Wnt-10a;wingless related MMTV integration site 10a.
NCBI Gene Id22409
Protein NameP70701
Description of TargetProtein Wnt-10a
Uniprot IDhttps://www.uniprot.org/uniprot/P70701
Protein Accession #NP_033544.1
Nucleotide Accession #NM_009518.2
Protein Size (# AA)417
Molecular Weight46 kDa
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