TXN Chemi-Luminescent ELISA Kit (Human) (OKCD04003)

Catalog No: OKCD04003

Size:96WELLS

Human TXN ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	81-103%	91-98%	83-99%	80-91%
  EDTA Plasma (n=5)	90-101%	84-101%	86-96%	98-105%
  Heparin Plasma (n=5)	98-105%	82-99%	90-102%	86-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Thioredoxin (Trx). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thioredoxin (Trx). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thioredoxin (Trx) level in the sample or standard.
• ELISA Kit Range: 0.14-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83-90	87
  EDTA Plasma (n=5)	82-93	88
  Heparin Plasma (n=5)	90-98	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin (Trx) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin (Trx) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • TXN Microplate: 96 Wells (12 x 8 Well strips)
  • TXN Lyophilized Standard: 2
  • 100X TXN Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.00549 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TXN
• Gene Full Name: thioredoxin
• Alias Symbols: ADF;ATL-derived factor;SASP;surface-associated sulphydryl protein;testicular tissue protein Li 199;thioredoxin;thioredoxin delta 3;TRDX;TRX;TRX1;Trx80;TXN delta 3.
• NCBI Gene Id: 7295
• Protein Name: Thioredoxin
• Description of Target: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
• Uniprot ID: P10599
• Protein Accession #: NP_001231867
• Nucleotide Accession #: NM_001244938