Catalog No: OKBB00563
Size:96 Wells
Price: $583.00
SKU
OKBB00563
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human Thioredoxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Thioredoxin has been precoated onto 96-well plates. Standards(E.coli, V2-V105) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Thioredoxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Thioredoxin amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)0.893.626.830.873.857.12
SD0.050.170.370.070.240.41
CV%5.64.75.486.25.8
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human Thioredoxin Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human Thioredoxin Standard2 x 10 ng/tube
100X Biotinylated Anti-Human Thioredoxin Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human Thioredoxin
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: E.coli; Immunogen sequence: V2-V105
DescriptionFor quantitative detection of human Thioredoxin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolTXN
Gene Full Namethioredoxin
Alias SymbolsADF, ATL-derived factor, DKFZp686B1993, MGC61975, SASP, Surface associated sulphydryl protein, Surface-associated sulphydryl protein, THIO_HUMAN, Thioredoxin, TRDX, TRX 1, Trx, TRX1, TXN, TXN protein
NCBI Gene Id7295
Protein NameThioredoxin
Description of TargetThioredoxin is a class of small redox proteins known to be present in all organisms. It is mapped to 9q31.3. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. They can also act as electron donors to peroxidases and ribonucleotide reductase. Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It plays a role in many important biological processes, including redox signaling. This gene also plays a central role in humans and is increasingly linked to medicine through their response to reactive oxygen species (ROS). VDUP1 is a key stress-responsive inhibitor of Thioredoxin activity in cardiomyocytes.
Uniprot IDP10599
Protein Accession #NP_001231867.1
Nucleotide Accession #NM_001244938.1
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