TRAF6 Antibody - middle region (OAGA04185)

Catalog No: OAGA04185

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A549
B: HeLa
C: HepG2
7.5% SDS PAGE
diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: BCL-1
7.5% SDS PAGE
diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

TRAF6 antibody [N2C2], Internal detects TRAF6 protein by western blot analysis.
A. 30 ug PC-12 whole cell extract
B. 30 ug Rat2 whole cell extract
7.5% SDS-PAGE
TRAF6 antibody [N2C2], Internal dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal diluted at 1:2000.

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using TRAF6 antibody at 1:500 dilution.

TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
TRAF6 antibody [N2C2], Internal diluted at 1:500.

TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TRAF6 antibody [N2C2], Internal diluted at 1:500.

TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Hoechst 33342 staining.

TRAF6 antibody [N2C2], Internal immunoprecipitates TRAF6 protein in IP experiments.
IP samples: K562 whole cell extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of TRAF6 protein by 4 ug TRAF6 antibody [N2C2], Internal
7.5 % SDS-PAGE
The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TRAF6. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.3 mg/ml
• Preservative: 0.025% ProClin 300
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Target Info

• Gene Symbol: TRAF6
• Gene Full Name: TNF receptor associated factor 6
• Alias Symbols: RNF85, MGC:3310
• NCBI Gene Id: 7189
• Protein Name: TNF receptor-associated factor 6
• Description of Target: The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. This protein also interacts with the transforming growth factor (TGF) beta receptor complex and is required for Smad-independent activation of the JNK and p38 kinases. This protein has an amino terminal RING domain which is followed by four zinc-finger motifs, a central coiled-coil region and a highly conserved carboxyl terminal domain, known as the TRAF-C domain. Two alternatively spliced transcript variants, encoding an identical protein, have been reported.
• Uniprot ID: Q9Y4K3
• Protein Accession #: NP_004611.1
• Nucleotide Accession #: NM_004620.3