Catalog No: OKEH03725
Size:96T
Price: $625.00
SKU
OKEH03725
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology TRAF6 ELISA Kit (Rat) (OKEH03725) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Traf6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Traf6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Traf6 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 15.6-1000pg/mL | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 7.92 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Rat TNF receptor-associated factor 6 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Traf6 |
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Gene Full Name | TNF receptor associated factor 6 |
Alias Symbols | E3 ubiquitin-protein ligase TRAF6;RING-type E3 ubiquitin transferase TRAF6;TNF receptor-associated factor 6;TNF receptor-associated factor 6, E3 ubiquitin protein ligase. |
NCBI Gene Id | 311245 |
Protein Name | B5DF45 |
Description of Target | TNF receptor-associated factor 6 |
Uniprot ID | https://www.uniprot.org/uniprot/B5DF45 |
Protein Accession # | NP_001101224.1 |
Nucleotide Accession # | NM_001107754.2 |
Protein Size (# AA) | 530 |
Molecular Weight | 60 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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