TRAF6 Antibody - C - terminal region (OAPB00191)

Catalog No: OAPB00191 (Formerly GWB-BA589C)

Size:100UG

Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 μg/mL in the presence (A) or absence (B) of 1 μg blocking peptide.

Immunofluorescence of TRAF6 in K562 cells with TRAF6 antibody at 20 ug/mL.

Immunocytochemistry of TRAF6 in K562 cells with TRAF6 antibody at 0.5 μg/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Porcine|Rat
• Product Format: Liquid. TRAF6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Additional Information: Positive Control: PC3 lysate, K562 lysate
• Reconstitution and Storage: TRAF6 antibody can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: TRAF6 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human TRAF6. The immunogen is located within the last 50 amino acids of TRAF6.
• Purification: Ion Exchange
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-TRAF6 (OAPB00191) antibody is Catalog # OEPB02287
• Application Info: TRAF6 antibody can be used for detection of TRAF6 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.
• Reference: Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.;
  Wu H. Assembly of post-receptor signaling complexes for the tumor necrosis factor superfamily. Adv. Protein Chem. 2004; 68:225-79.;
  Wajant H and Scheurich P. Analogies between Drosophila and mammalian TRAF pathways. Prog. Mol. Subcell. Biol. 2004; 34:47-72.;
  Kobayashi T, Walsh PT, Walsh MC, et al. TRAF6 is a critical factor for dendritic cell maturation and development. Immunity 2003; 19:353-63.

Target Info

• Gene Symbol: TRAF6
• Gene Full Name: TNF receptor associated factor 6
• Alias Symbols: E3 ubiquitin-protein ligase TRAF6;interleukin-1 signal transducer;MGC:3310;RING finger protein 85;RING-type E3 ubiquitin transferase TRAF6;RNF85;TNF receptor-associated factor 6;TNF receptor-associated factor 6, E3 ubiquitin protein ligase.
• NCBI Gene Id: 7189
• Protein Name: TNF receptor-associated factor 6|TNF receptor-associated factor 6 [Homo sapiens]
• Description of Target: E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.
• Uniprot ID: Q9Y4K3
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004611
• Nucleotide Accession #: NM_004620.3; NM_145803.2