TNFSF13B ELISA Kit (Mouse) (OKCD07585)

Catalog No: OKCD07585

Size:96WELLS

TNFSF13B ELISA Kit (Mouse) (OKCD07585) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Activating Factor (BAFF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell Activating Factor (BAFF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell Activating Factor (BAFF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of B-Cell Activating Factor (BAFF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 12.5-800pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell Activating Factor (BAFF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell Activating Factor (BAFF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • TNFSF13B Microplate: 96 Wells (12 x 8 Well strips)
  • TNFSF13B Lyophilized Standard: 2
  • 100X TNFSF13B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 4.9pg/mL

Target Info

• Gene Symbol: Tnfsf13b
• Gene Full Name: tumor necrosis factor (ligand) superfamily, member 13b
• Alias Symbols: b cell-activating factor;BAF;BAFF;B-cell-activating factor;BL;BLyS;D8Ertd387;D8Ertd387e;TAL;TALL1;TALL-1;THANK;TNFSF20;Tnlg7a;tumor necrosis factor ligand 7a;tumor necrosis factor ligand superfamily member 13B;zTNF;zTNF4.
• NCBI Gene Id: 24099
• Protein Name: Tumor necrosis factor ligand superfamily member 13B
• Description of Target: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
• Uniprot ID: Q9WU72
• Protein Accession #: NP_001334238.1
• Nucleotide Accession #: NM_001347309.1