TNFSF13B Antibody (OAED00325)

Catalog No: OAED00325

Size:100 ug

Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 105) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1 ug/ml of MAb to BAFF (mouse) (5A8). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

Product Info

• Predicted Species Reactivity: Mouse
• Clonality: Monoclonal
• Clone: 5A8
• Isotype: IgG1
• Host: Rat
• Application: FC, WB
• Reconstitution and Storage: Long Term Storage : -20C
  Short Term Storage : +4C
  Handling : Avoid freeze/thaw cycles.
  Stable for at least 1 year after receipt when stored at -20C.
• Immunogen: Rat RBL cells expressing mouse BAFF.
• Concentration: 1mg/ml
• Specificity: Recognizes mouse BAFF.
• Formulation: Liquid. In PBS containing 0.02% sodium azide.
• Purity: >95% (SDS-PAGE)
• Application Info: ELISA (capture)
  Flow Cytometry (excellent)
  Western Blot
• Reference: TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004), Abstract;
  

Target Info

• Gene Symbol: TNFSF13B
• Gene Full Name: Tumor necrosis factor ligand superfamily member 13B
• Alias Symbols: DTL, BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNLG7A, TNFSF20
• NCBI Gene Id: 10673
• Protein Name: Tumor necrosis factor ligand superfamily member 13B
• Uniprot ID: Q9Y275
• Protein Accession #: NP_001139117.1; NP_006564.1
• Nucleotide Accession #: NM_001145645.2; NM_006573.4