TNFSF13B Antibody (OAED00324)

Catalog No: OAED00324

Size:100UG

Method: HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse BAFF (thick line). Cells (5 x 105) were incubated on ice for 30 min in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1 ug/ml of MAb to BAFF (mouse) (1C9). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

Product Info

• Predicted Species Reactivity: Mouse
• Clonality: Monoclonal
• Clone: 1C9
• Isotype: IgG1
• Host: Rat
• Application: Enzyme-linked immunosorbent assay|Flow cytometry
• Reconstitution and Storage: Long Term Storage : -20C
  Short Term Storage : +4C
  Handling : Avoid freeze/thaw cycles.
  Stable for at least 1 year after receipt when stored at -20C.
• Immunogen: Rat RBL cells expressing mouse BAFF.
• Concentration: 1mg/ml
• Specificity: Recognizes mouse BAFF.
• Formulation: Liquid. In PBS containing 0.02% sodium azide.
• Purity: >95% (SDS-PAGE).
• Application Info: ELISA (detection)
  Flow Cytometry

Target Info

• Reference: TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004), Abstract;
  
• Gene Symbol: Tnfsf13b
• Gene Full Name: tumor necrosis factor (ligand) superfamily, member 13b
• Alias Symbols: b cell-activating factor;BAF;BAFF;B-cell-activating factor;BL;BLyS;D8Ertd387;D8Ertd387e;TAL;TALL1;TALL-1;THANK;TNFSF20;Tnlg7a;tumor necrosis factor ligand 7a;tumor necrosis factor ligand superfamily member 13B;zTNF;zTNF4.
• NCBI Gene Id: 24099
• Protein Name: Tumor necrosis factor ligand superfamily member 13B
• Description of Target: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
• Uniprot ID: Q9WU72
• Protein Accession #: NP_001139117.1; NP_006564.1
• Nucleotide Accession #: NM_001145645.2; NM_006573.4