TNFRSF11B Antibody (OAED00350)

Catalog No: OAED00350

Size:100 ug

Figure: FACS analysis of cells with MAb to OPG (human) (Bony-1). Method: HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 ug/ml of MAb to OPG (human) (Bony-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: Bony-1
• Isotype: IgG2a
• Host: Rat
• Application: FC, IP
• Reconstitution and Storage: Long Term Storage : -20C
  Short Term Storage : +4C
  Handling : Avoid freeze/thaw cycles.
  Stable for at least 1 year after receipt when stored at -20C.
• Immunogen: Recombinant human OPG (osteoprotegerin) cysteine-rich region (aa 22-202) (Prod. No. ALX-522-007).
• Concentration: 1mg/ml
• Specificity: Recognizes human OPG.
• Source: Purified from concentrated hybridoma tissue culture supernatant.
• Formulation: Liquid. In PBS containing 0.02% sodium azide.
• Purity: ≥95% (SDS-PAGE)
• Application Info: ELISA
  Flow Cytometry (1:1,000)
  Immunoprecipitation (1:200)
• Reference: Osteoprotegerin is a soluble decoy receptor for tumor necrosis factor-related apoptosis-inducing ligand/Apo2 ligand and can function as a paracrine survival factor for human myeloma cells: C.M. Shipman and P.I. Croucher; Cancer Res. 63, 912 (2003), Abstract; Full Text
  Osteoprotegerin: U. Feige; Ann. Rheum. Dis. 60, Suppl 3, iii81 (2001), Abstract; Full Text

Target Info

• Gene Symbol: TNFRSF11B
• Gene Full Name: Tumor necrosis factor receptor superfamily member 11B
• Alias Symbols: OPG, TR1, OCIF, PDB5
• NCBI Gene Id: 4982
• Protein Name: Tumor necrosis factor receptor superfamily member 11B
• Uniprot ID: O00300
• Protein Accession #: NP_002537.3
• Nucleotide Accession #: NM_002546.3