TNFRSF11B Antibody - middle region (OAAB10038)

Catalog No: OAAB10038

Size:400ul

TNFRSF11B Antibody (Center) (Cat. #OAAB10038) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TNFRSF11B antibody detected the TNFRSF11B protein (arrow).

TNFRSF11B Antibody (Center) (OAAB10038)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TNFRSF11B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of TNFRSF11B Antibody (Center) (Cat#OAAB10038) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IF, IHC
• Additional Information: Precautions: TNFRSF11B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis
  Cellular Location: Secreted.
  Tissue Location: Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This TNFRSF11B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the Central region of human TNFRSF11B.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IF: 1:10~50
  IHC-P: 1:10~50

Target Info

• Gene Symbol: TNFRSF11B
• Alias Symbols: OPG, TR1, OCIF, PDB5
• NCBI Gene Id: 4982
• Protein Name: Tumor necrosis factor receptor superfamily member 11B
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O00300
• Protein Accession #: NP_002537.3
• Molecular Weight: 46026 Da