Catalog No: OKBB00253 (Formerly GWB-ZZD028 )
Size:96 Tests
Price: $466.00
SKU
OKBB00253
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from chicken specific for TGF beta 1 has been precoated onto 96-well plates. Standards(CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from chicken specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TGF beta 1 amount of sample captured in plate.
ELISA Kit Range15.6 pg/ml - 1,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)135264521152270543
St. Dev.5.9413.7318.87.617.332.04
%CV4.45.203.6056.405.90
ELISA Kit Component
ComponentAmount
Lyophilized recombinant human TGFB1 standard10 ng/tube x 2
Anti-human TGFB1 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti-human TGFB1 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional InformationRange: 15.6pg/ml-1000pg/m
::Principle: Aviva’s human TGFβ1 ELISA Kit was based on standard sandwich
enzyme-linked immune-sorbent assay technology. Human TGFβ1
specific-specific polyclonal antibodies were precoated onto 96-well plates. The
human specific detection polyclonal antibodies were biotinylated. The test
samples and biotinylated detection antibodies were added to the wells
subsequently and then followed by washing with PBS or TBS buffer.
Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were
washed away with PBS or TBS buffer. HRP substrate TMB was used to
visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a
blue color product that changed into yellow after adding acidic stop solution.
The density of yellow is proportional to the human TGFβ1 amount of sample
captured in plate.
::Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion.TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein.The standard product used in this kit is recombinant TGFβ1 with the molecular mass of 25KDa
::Activating Reagent: TGFβ1 is mostly contained as inactive form in samples, please activate it before assay. Don’t activate recombinant TGFβ1.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H2O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml.
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum, plasma (EDTA) and urine
Sensitivity<1 pg/ml
Predicted Homology Based on Immunogen SequenceCross-reactivates with TGFβ2, TGFβ3, TGFβ5 <1%.
Reference1. Sporn, M. B.; Roberts, A. B.; Wakefield, L. M.; Assoian, R. K. Transforming growth factor-beta: biological function
and chemical structure. Science 233: 532-534, 1986.
2. Blanchette, F.; Day, R.; Dong, W.; Laprise, M.-H.; Dubois, C. M. TGF-beta-1 regulates gene expression of its own
converting enzyme furin. J. Clin. Invest. 99: 1974-1983, 1997.
3. Janssens, K.; Gershoni-Baruch, R.; Guanabens, N.; Migone, N.; Ralston, S.; Bonduelle, M.; Lissens, W.; Van
Maldergem, L.; Vanhoenacker, F.; Verbruggen, L.; Van Hul, W. Mutations in the gene encoding the
latency-associated peptide of TGF-beta-1 cause Camurati-Engelmann disease. Nature Genet. 26: 273-275, 2000.
4. Li, A. G.; Wang, D.; Feng, X.-H.; Wang, X.-J. Latent TGF-beta-1 overexpression in keratinocytes results in a severe
psoriasis-like skin disorder. EMBO J. 23: 1770-1781, 2004.
5. Li, B.; Khanna, A.; Sharma, V.; Singh, T.; Suthanthiran, M.; August, P. TGF-beta-1 DNA polymorphisms, protein
levels, and blood pressure. Hypertension 33: 271-275, 1999.
6. Saito, T.; Kinoshita, A.; Yoshiura, K.; Makita, Y.; Wakui, K.; Honke, K.; Niikawa, N.; Taniguchi, N. Domain-specific
mutations of a transforming growth factor (TGF)-beta 1 latency-associated peptide cause Camurati-Engelmann
disease because of the formation of a constitutively active form of TGF-beta 1. J. Biol. Chem. 276: 11469-11472,
2001.
SpecificityNatural and recombinant human TGF beta1
ImmunogenExpression system for standard: CHO; Immunogen sequence: A279-S390
Gene SymbolTGFB1
Gene Full Nametransforming growth factor, beta 1
Alias SymbolsCamurati-Engelmann disease, CED, DPD1, LAP, Latency-associated peptide, TGF beta 1 protein, TGF-beta-1, TGFB, tgfb1, TGFB1_HUMAN, TGFbeta, Transforming growth factor beta 1
NCBI Gene Id7040
Protein NameTransforming growth factor beta-1
Description of TargetTransforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion.TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein.The standard product used in this kit is recombinant TGFβ 1 with the molecular mass of 25KDa.
Uniprot IDP01137
Protein Accession #NP_000651.3
Nucleotide Accession #NM_000660.5
Write Your Own Review
You're reviewing:TGFB1 ELISA Kit (Human) (OKBB00253)
Your Rating
We found other products you might like!