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Catalog No: OKAA00026-96W
Size:96 Wells
Price: $425.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit ReproducibilityThe coefficient of variation of both intra-assay and inter-assay were less than 10%.
ELISA Kit Component
2 vials human TGF-beta1 Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human TGF-beta1 antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody
Additional InformationSample Type: cell culture supernates, serum, and plasma
::Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human TGF-?1 (8x12)
2. 2 vials human TGF-?1 Standard lyophilized, 2000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human TGF-?1 antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 1 vial 1N HCl
12. 1 vial 1N NaOH
13. 3 pieces Adhesive Films
14. package insert
Sample Typecell culture, supernates, serum, and plasma
Sensitivity15 pg/ml
Predicted Homology Based on Immunogen SequenceHuman
SpecificityThis assay recognizes both natural and recombinant human sICAM-1. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: VCAM-1, L-Selectin, Siglec-2, Siglec-3, Siglec-5, ICAM-2, ICAM-3 ,
Recombinant mouse: JAM-A, JAM-B, JAM-C, ICAM-2, ICAM-5
Assay InfoAssay Method: Quantitative Sandwich ELISA
Gene SymbolTGFB1
Gene Full Nametransforming growth factor beta 1
Alias SymbolsCED, LAP, DPD1, TGFB, IBDIMDE, TGFbeta, TGF-beta1
NCBI Gene Id7040
Protein NameTransforming growth factor beta-1
Description of TargetThis gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
Uniprot IDP01137
Protein Accession #NP_000651.3
Nucleotide Accession #NM_000660.5
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