TGFB1 ELISA Kit (Human) : 96 Wells (OKAA00026-96W)

Catalog No: OKAA00026-96W

Size:96 Wells

Standard curve of TGFB1 protein using OKAA00026_96W

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  2 vials human TGF-beta1 Standard lyophilized, 2000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human TGF-beta1 antibody
  2 vials Streptavidin-HRP solution,
  1 bottle Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  1 bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution
  2 vials human TNF-alpha Standard lyophilized, 4000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human TNF-alpha antibody

• Additional Information: Sample Type: cell culture supernates, serum, and plasma
  Reagents: REAGENTS
  1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human TGF-?1 (8x12)
  2. 2 vials human TGF-?1 Standard lyophilized, 2000 pg/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-human TGF-?1 antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 1 vial 1N HCl
  12. 1 vial 1N NaOH
  13. 3 pieces Adhesive Films
  14. package insert
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 15 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This assay recognizes both natural and recombinant human sICAM-1. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: VCAM-1, L-Selectin, Siglec-2, Siglec-3, Siglec-5, ICAM-2, ICAM-3 ,
  Recombinant mouse: JAM-A, JAM-B, JAM-C, ICAM-2, ICAM-5
  :
• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TGFB1
• Gene Full Name: transforming growth factor beta 1
• Alias Symbols: CED, LAP, DPD1, TGFB, IBDIMDE, TGFbeta, TGF-beta1
• NCBI Gene Id: 7040
• Protein Name: Transforming growth factor beta-1
• Description of Target: This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
• Uniprot ID: P01137
• Protein Accession #: NP_000651.3
  
• Nucleotide Accession #: NM_000660.5