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Catalog No: OABB00182 (Formerly GWB-BBP105)
Size:100UG
Price: $432.00
SKU
OABB00182
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Datasheets/ManualsPrintable datasheet for SKP2 Polyclonal Antibody (OABB00182)
Product Info
Tested Species ReactivityHuman, Rat
Predicted Species ReactivityHuman|Mouse|Rat
Product FormatLyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
ClonalityPolyclonal
ClonePolyclonal
IsotypeRabbit IgG
HostRabbit
ApplicationImmunohistochemistry|Western blot
Additional InformationNotes: Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
::Background: The F box protein Skp2(S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.
Reconstitution and Storage2°C to 8°C|-20°C
ImmunogenPolypeptide
PurificationAffinity Purified
Predicted Homology Based on Immunogen SequenceHuman; Mouse; Rat
Concentration500 ug/ml
SpecificityNo cross reactivity with other proteins.
Application InfoWestern blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
Reference1. Kitagawa, M.; Lee, S. H.; McCormick, F. : Skp2 suppresses p53-dependent apoptosis by inhibiting p300. Molec. Cell 29: 217-231, 2008.
2. Latres, E.; Chiarle, R.; Schulman, B. A.; Pavletich, N. P.; Pellicer, A.; Inghirami, G.; Pagano, M. : Role of the F-box protein Skp2 in lymphomagenesis. Proc. Nat. Acad. Sci. 98: 2515-2520, 2001.
Storage BufferEach vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
DescriptionRabbit IgG polyclonal antibody for S-phase kinase-associated protein 2(SKP2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Gene SymbolSKP2
Gene Full NameS-phase kinase associated protein 2
Alias SymbolsCDK2/cyclin A-associated protein p45;Cyclin-A/CDK2-associated protein p45;FBL1;F-box protein Skp2;F-box/LRR-repeat protein 1;FBXL1;FLB1;p45;p45skp2;S-phase kinase-associated protein 2;S-phase kinase-associated protein 2, E3 ubiquitin protein ligase.
NCBI Gene Id6502
Protein NameS-phase kinase-associated protein 2
Description of TargetSubstrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.
Uniprot IDQ13309
Molecular Weight47761 MW
  1. What is the species homology for "SKP2 Polyclonal Antibody (OABB00182)"?

    The tested species reactivity for this item is "Human, Rat". This antibody is predicted to have homology to "Human|Mouse|Rat".

  2. How long will it take to receive "SKP2 Polyclonal Antibody (OABB00182)"?

    This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".

  3. What buffer format is "SKP2 Polyclonal Antibody (OABB00182)" provided in?

    This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "SKP2 Polyclonal Antibody (OABB00182)"?

    This target may also be called "CDK2/cyclin A-associated protein p45;Cyclin-A/CDK2-associated protein p45;FBL1;F-box protein Skp2;F-box/LRR-repeat protein 1;FBXL1;FLB1;p45;p45skp2;S-phase kinase-associated protein 2;S-phase kinase-associated protein 2, E3 ubiquitin protein ligase." in publications.

  5. What is the shipping cost for "SKP2 Polyclonal Antibody (OABB00182)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "SKP2 Polyclonal Antibody (OABB00182)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "SKP2 Polyclonal Antibody (OABB00182)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "47761 MW".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "SKP2 Polyclonal Antibody (OABB00182)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "SKP2"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "SKP2"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "SKP2"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "SKP2"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "SKP2"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "SKP2"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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