SERPINE1 ELISA Kit (Rat) (OKCD06430)

Catalog No: OKCD06430

Size:96WELLS

SERPINE1 ELISA Kit (Rat) (OKCD06430) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plasminogen Activator Inhibitor 1 (PAI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Plasminogen Activator Inhibitor 1 (PAI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SERPINE1 Microplate: 96 Wells (12 x 8 Well strips)
  • SERPINE1 Lyophilized Standard: 2
  • 100X SERPINE1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: < 0.117ng/mL

Target Info

• Gene Symbol: Serpine1
• Gene Full Name: serpin family E member 1
• Alias Symbols: endothelial plasminogen activator inhibitor;PAI;Pai1;PAI-1;PAI1A;Pai1aa;Planh;plasminogen activator inhibitor 1;RATPAI1A;serine (or cysteine) peptidase inhibitor, clade E, member 1;serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1;serine (or cysteine) proteinase inhibitor, clade E, member 1;serine (or cysteine) proteinase inhibitor, member 1;serpin E1;serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1.
• NCBI Gene Id: 24617
• Protein Name: Plasminogen activator inhibitor 1
• Description of Target: Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence (By similarity). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).
• Uniprot ID: P20961
• Protein Accession #: NP_036752.1
• Nucleotide Accession #: NM_012620.1