S100A8 ELISA Kit (Mouse) (OKEH03410)

Catalog No: OKEH03410

Size:96T

Mouse S100A8 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology S100A8 ELISA Kit (Mouse) (OKEH03410) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100a8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for S100a8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S100a8 captured in well.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 4.6% (n = 20)
  Mean Inter-assay CV%: <= 7.1% (n = 20)
• ELISA Kit Component:
  • Anti-S100a8 Microplate: 96 Wells (12 x 8 Well strips)
  • S100a8 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated S100a8 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.334 ng/mL
• Specificity: Natural and recombinant Mouse Protein S100-A8
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: S100a8
• Gene Full Name: S100 calcium binding protein A8 (calgranulin A)
• Alias Symbols: 60B8A;60B8Ag;AI323541;B8A;B8Ag;Ca;Caga;calgranulin-A;CFA;CFAg;chemotactic cytokine CP-10;chemotactic S100 protein;CP-1;CP-10;leukocyte L1 complex light chain;migration inhibitory factor-related protein 8;MR;MRP8;MRP-8;p;p8;pro-inflammatory S100 cytokine;protein S100-A8;S100 calcium-binding protein A8.
• NCBI Gene Id: 20201
• Protein Name: P27005
• Description of Target: Protein S100-A8
• Uniprot ID: https://www.uniprot.org/uniprot/P27005
• Protein Accession #: NP_038678.1
• Nucleotide Accession #: NM_013650.2
• Protein Size (# AA): 89
• Molecular Weight: 10 kDa