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Datasheets/Manuals | Printable datasheet for anti-S100A8 (OAAD00030) antibody |
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Predicted Species Reactivity | Mouse, Rat, Monkey, baboon, Swine, Dog, Cat Rabbit, Guinea Pig |
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Product Format | Concentrated |
Clonality | Monoclonal |
Clone | B314.1 (MAC 387) |
Isotype | IgG1 |
Host | Mouse |
Application | IHC |
Additional Information | Recommended Positive Control: Tonsil, Lymph Node or Spleen Cellular Localization: cytoplasmic |
:: | Presentation: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5. Aliquoting Instructions: GENERAL INSTRUCTIONS: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. GENERAL INFO: When diluting for immunohistochemistry, ELISA or western blot, make the dilution in Antibody Diluting Buffer. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability. |
Reconstitution and Storage | Store at 2 - 8C for short term, freeze under -20C for long term storage. |
Immunogen | Affinity purified monocyte membrance preparation. |
Specificity | This antibody reacts with macrophage L1 protein, also known as calprotectin, calgranulin, or cystic fibrosis antigen. |
Application Info | Immunohistochemistry 1:50-1:100. |
Protocol Information | Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. This antibody may be used at a dilution 1:50-1:100 in IHC. When using routinely processed tissues, predigestion with trypsin @ 1 mg/ml PBS, 10 mins. at 37 C. or a high temperature antigenic unmasking technique (boiling tissue sections in 10 mM citrate, pH 6.0 for 10-20 mins., folowed by cooling to RT for 10-20 mins.)) is required. The optimal conditions should be determined by the individual laboratory. |
Reference | 1. Brandzaeg P, Jones DB, Flavell DJ, Fagerhold MK. MAC 387 antibody and detection of formalin resistant myelomonocytic L1 antigen. J Clin Pathol 41: 963-70, 1988. 2. Brandtzaeg P, Dale I, Gabrielsen T-Q The leucocyte protein L1 (calprotein): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathology; 1992. 3. Anderson, CB; et al. Apmis, 1994, 102(1): 23-27. |
Gene Symbol | S100A8 |
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Gene Full Name | Protein S100-A8 |
Alias Symbols | P8, MIF, NIF, CAGA, CFAG, CGLA, L1Ag, MRP8, CP-10, MA387, 60B8AG |
NCBI Gene Id | 6279 |
Protein Name | Protein S100-A8 |
Description of Target | Monoclonal Mouse Anti-Human Macrophage/L1 Protein/Calprotectin (Myeloid/Histiocyte Antigen) |
Uniprot ID | P05109 |
Protein Accession # | NP_002955.2 |
Nucleotide Accession # | NM_002964.4 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "S100A8 Antibody (OAAD00030)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse, Rat, Monkey, baboon, Swine, Dog, Cat Rabbit, Guinea Pig".
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How long will it take to receive "S100A8 Antibody (OAAD00030)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "S100A8 Antibody (OAAD00030)" provided in?
This item is provided in "Concentrated".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "S100A8 Antibody (OAAD00030)"?
This target may also be called "P8, MIF, NIF, CAGA, CFAG, CGLA, L1Ag, MRP8, CP-10, MA387, 60B8AG" in publications.
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What is the shipping cost for "S100A8 Antibody (OAAD00030)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "S100A8 Antibody (OAAD00030)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "S100A8 Antibody (OAAD00030)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "S100A8 Antibody (OAAD00030)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "S100A8"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "S100A8"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "S100A8"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "S100A8"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "S100A8"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "S100A8"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.