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Datasheets/Manuals | Printable datasheet for anti-RHOA (OAAB21952) antibody |
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Predicted Species Reactivity | Human, Mouse |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB, IF, ICC, IHC-P |
Additional Information | Function: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Predicted Homology Based on Immunogen Sequence | Human, Mouse |
Preservative | 0.03% Proclin300 and 50% glycerol |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
Application Info | FC: 1:20~1:100 WB: 1:100~1:500 IHC-P: 1:100~1:500 |
Gene Symbol | RHOA |
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Gene Full Name | ras homolog family member A |
Alias Symbols | ARHA, ARH12, RHO12, EDFAOB, RHOH12 |
NCBI Gene Id | 387 |
Description of Target | This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. |
Uniprot ID | P61586 |
Protein Accession # | NP_001300870.1 |
Nucleotide Accession # | NM_001313941.1 |
Molecular Weight | 22 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "RHOA Antibody (OAAB21952)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
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How long will it take to receive "RHOA Antibody (OAAB21952)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "RHOA Antibody (OAAB21952)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "RHOA Antibody (OAAB21952)"?
This target may also be called "ARHA, ARH12, RHO12, EDFAOB, RHOH12" in publications.
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What is the shipping cost for "RHOA Antibody (OAAB21952)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "RHOA Antibody (OAAB21952)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "RHOA Antibody (OAAB21952)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "22 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "RHOA Antibody (OAAB21952)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "RHOA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "RHOA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "RHOA"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "RHOA"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "RHOA"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "RHOA"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.