RHOA Antibody (OAAB21952)

• Product Number: OAAB21952
• Name: RHOA Antibody (OAAB21952)
• Molecular Weight: 22 kDa
• NCBI Gene Id: 387
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: ras homolog family member A
• Alias Symbols: ARHA, ARH12, RHO12, EDFAOB, RHOH12
• Product Format: Liquid
• Description of Target: This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
• Application Info: FC: 1:20~1:100
  WB: 1:100~1:500
  IHC-P: 1:100~1:500
• Formulation: 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
• Uniprot ID: P61586
• Protein Accession #: NP_001300870.1
• Nucleotide Accession #: NM_001313941.1
• Preservative: 0.03% Proclin300 and 50% glycerol
• Gene Symbol: RHOA
• Predicted Species Reactivity: Human, Mouse
• Application: WB, IF, ICC, IHC-P
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Image 1: Human Hela Cells
  
  Hela cells probed with RhoA Antibody , Unconjugated (OAAB21952) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
• Image 2: Human Skin Cells
  
  Formalin-fixed and paraffin embedded human skin labeled with RhoA Antibody ( (OAAB21952), Unconjugated 1:400 followed by conjugation to the secondary antibody and DAB staining
• Image 3: Human Colon Carcinoma Cells, Rat Brain Cells
  
  L1 human colon carcinoma lysates L2 rat brain lysates probed with RhoA Antibody , Unconjugated (OAAB21952) at 1:200 overnight at 4C. Followed by conjugation to secondary antibody at 1:3000 for 90 min at 37C. Predicted band 21kD. Observed band size: 21kD.