Size:96 Wells
$600.00
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SKU
OKCA00795
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RAB35 ELISA Kit (Human) (OKCA00795)

Catalog#: OKCA00795
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3.5 Hours
Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9289-96
1:29591-99
1:49792-102
1:89085-96
Kit Principle Aviva Systems Biology RAB35 ELISA Kit (Human) (OKCA00795) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAB35 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAB35 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RAB35 captured in the well.
Kit Range 47 pg/mL-3,000 pg/mL
Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9689-102
EDTA Plasma (n=4)9490-98
Kit Reproducibility Intra-assay Precision: 3 samples with known low, middle and high level RAB35 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level RAB35 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Component
ComponentAmount
RAB35 Microplate96 Wells (12 x 8 Well strips)
RAB35 Lyophilized Standard2
100X Biotinylated RAB35 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity 11.7 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol RAB35
Official Gene Full Name RAB35, member RAS oncogene family
Alias Symbols GTP-binding protein RAY, H-ray, RAB1C, Ras-related protein Rab-1C, Ras-related protein Rab-35, RAY
NCBI Gene Id 11021
Protein Name Ras-related protein Rab-35
Description of Target The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth.
Swissprot Id Q15286
Protein Accession # NP_001161078.1
Nucleotide Accession # NM_001167606.1
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express RAB35.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express RAB35.
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