RAB35 Antibody - C-terminal region (OAAB18161)

Catalog No: OAAB18161

Size:400ul

Western blot analysis of lysates from Hela, U-87 MG, rat PC-12 cell line, huamn brain and mouse brain tissue lysate (from left to right), using RAB35 Antibody (C-term) (Cat. #OAAB18161).
OAAB18161 was diluted at 1:1000 at each lane.
A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Lysates at 35ug per lane.
Dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded H.small intestine section using RAB35 Antibody (C-term)(Cat#OAAB18161). OAAB18161 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P
  Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin- coated vesicle. Endosome Melanosome. Note=Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065).
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RAB35 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 166-199 amino acids from the C-terminal region of human RAB35.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Intended Use: RAB35 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: IHC-P: 1:25
  WB: 1:1000
• Reference: Zhu A.X., et al.Biochem. Biophys. Res. Commun. 205:1875-1882(1994).
  Puhl H.L. III, et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
  Kalnine N., et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
  Halleck A., et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
  Ota T., et al.Nat. Genet. 36:40-45(2004).

Target Info

• Gene Symbol: RAB35
• Alias Symbols: RAY, H-ray, RAB1C
• NCBI Gene Id: 11021
• Description of Target: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4, 5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth.
• Uniprot ID: Q15286, Q5U316, Q6PHN9
• Molecular Weight: 23 kDa