PTPRC Antibody (OAEE00256)

Catalog No: OAEE00256

Size:100UG

Immunohistochemistry staining of tonsil (paraffin-embedded sections) with anti-CD45RB (MEM-55).

Product Info

• Predicted Species Reactivity: Human|Non-human Primate
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MEM-55
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Human thymocytes and T lymphocytes.
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-55 recognizes a siliadase-sensitive epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
  HLDA III; WS Code NL 358
  HLDA IV; WS Code NL 2
  HLDA V; WS Code T T-151
  HLDA V; WS Code T T-CD45.08
• Purity: > 95% (by SDS-PAGE)
• Application Info: Flow Cytometry 2 μg/ml
  Immunohistochemistry (paraffin sections) No pre-treatment of tissue sections is essential.
• Reference: Koethe S, Zander L, Koster S, Annan A, Ebenfelt A, Spencer J, Bemark M: Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. 2011 Jul;90(1):5-19.
  Horejsi V, Angelisova P, Bazil V, Kristofova H, Stoyanov S, Stefanova I, Hausner P, Vosecky M, Hilgert I: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha). 1988;34(1):23-34.
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).
  Desharnais P, Dupere-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19
  Bijian K, Zhang L, Shen SH: Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol Immunol. 2007 Jul;44(15):3682-90.
  Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176(6):3417-25.
  Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis. Brain Pathol. 2006 Oct;16(4):256-65.
  Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood. 2005 Apr 15;105(8):3295-302.
  Townsend KP, Vendrame M, Ehrhart J, Faza B, Zeng J, Town T, Tan J: CD45 isoform RB as a molecular target to oppose lipopolysaccharide-induced microglial activation in mice. Neurosci Lett. 2004 May 13;362(1):26-30.

Target Info

• Gene Symbol: PTPRC
• Gene Full Name: Receptor-type tyrosine-protein phosphatase C
• Alias Symbols: LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180
• NCBI Gene Id: 5788
• Protein Name: Receptor-type tyrosine-protein phosphatase C
• Description of Target: CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
• Uniprot ID: P08575
• Protein Accession #: NP_002829.3; NP_563578.2
• Nucleotide Accession #: NM_002838.4; NM_080921.3